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    2.

    A researcher isolates an enzyme from a thermophilic bacterium and finds that its activity dramatically decreases when incubated at 25ยฐC compared to its optimal temperature of 70ยฐC. This decrease in activity is MOST likely due to:

    A

    Increased substrate affinity at lower temperatures.

    B

    Changes in the enzyme's three-dimensional structure affecting the active site.

    C

    A decrease in the activation energy of the catalyzed reaction.

    D

    An increase in the concentration of competitive inhibitors at lower temperatures.

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    8.

    Metabolic pathways are often regulated through feedback inhibition. This means:

    A

    The substrate of a pathway stimulates the first enzyme.

    B

    Enzymes are constantly synthesized regardless of product concentration.

    C

    The end product of a pathway inhibits an earlier enzyme in the pathway.

    D

    Enzymes are degraded rapidly after catalyzing a reaction.

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