The Question is from neet-botany cell-biology , and it was created by the author D VINOD

Question

Which statement BEST describes the role of inclusion bodies in recombinant protein production?

D VINOD · Accepted Answer

## CORE INFORMATION
✓ **Answer**: Option B
## CONCEPT FRAMEWORK
 **Primary Concept**: Recombinant protein production and purification
 **Related Topics**: Inclusion bodies, protein folding, proteolysis, purification techniques
 **Prerequisites**: Basic understanding of protein structure and function, recombinant DNA technology.
## SOLUTION PATHWAY
**Given Information**:
  The question asks about the role of inclusion bodies in recombinant protein production.
  The options describe different potential roles.
**Method & Steps**:
 1. Analyze each option to determine its accuracy regarding inclusion bodies.
  Reasoning: Inclusion bodies are insoluble aggregates of misfolded proteins that often form in bacterial cells during recombinant protein expression.  They are not desirable but are a common occurrence.
 2. Evaluate the role of inclusion bodies in purification and refolding.
  Working: Inclusion bodies simplify the initial purification steps because they can be easily separated from the soluble cellular components by centrifugation. However, the proteins within inclusion bodies are misfolded and inactive, requiring a refolding step to regain their native structure and function.
 3. Compare this with other roles.
  Key Point: Inclusion bodies do *not* prevent proteolysis, enhance expression levels, or facilitate secretion. They are a consequence of misfolding and aggregation.
 3. Select the best option.
  Result: Option B accurately describes the role of inclusion bodies: they simplify purification (initial steps) but necessitate subsequent refolding steps to restore protein activity.
  Verification: Option B aligns with the established understanding of inclusion body formation and processing in recombinant protein production.
 ## OPTION ANALYSIS
A :x: They prevent proteolysis of the recombinant protein.  (Incorrect: Inclusion bodies don't prevent proteolysis; they often result from it or contribute to it.)
B ✓: They simplify purification but require refolding steps. (Correct:  Inclusion bodies are easily separated, but the proteins must be refolded.)
C :x: They enhance the expression levels of the recombinant protein. (Incorrect:  Inclusion body formation is typically associated with *reduced* yield of correctly folded protein.)
D :x: They facilitate secretion of the recombinant protein. (Incorrect: Inclusion bodies are intracellular aggregates; they do not facilitate secretion.)
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing inclusion bodies with a beneficial aspect of recombinant protein production: They are a challenge to overcome, not a desirable outcome.
  2.Overlooking the refolding step:  Purification from inclusion bodies is only the first step.  Refolding is crucial to achieve the functional protein.
 **Quick Tips**:
  1.Remember the key feature: Insoluble aggregates require purification and refolding.
  2.Associate inclusion bodies with misfolded proteins and the need for downstream processing.

D VINOD · Answer

They prevent proteolysis of the recombinant protein.

D VINOD · Answer

The correct Option is "They simplify purification but require refolding steps."

D VINOD · Answer

They enhance the expression levels of the recombinant protein.

D VINOD · Answer

They facilitate secretion of the recombinant protein.

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