The Question is from neet-botany cell-biology , and it was created by the author K SURESH KUMAR

Question

The formation of inclusion bodies is often associated with:

K SURESH KUMAR · Accepted Answer

## CORE INFORMATION
✓ **Answer**: [A] 
## CONCEPT FRAMEWORK
	**Primary Concept**: Protein misfolding and aggregation
	**Related Topics**: Recombinant protein expression, Chaperone proteins, Protein solubility
	**Prerequisites**: Understanding of protein structure and function, basics of recombinant DNA technology
## SOLUTION PATHWAY
**Given Information**:
		- Inclusion bodies are aggregates of misfolded proteins.
		- Often observed in the production of recombinant proteins in bacterial systems.
**Method & Steps**:
	1. Consider the conditions under which proteins are expressed in prokaryotic systems.
		Reasoning: High expression rates can lead to an abundance of proteins being synthesized quickly, which outpaces the cellular machinery's ability to fold them properly.
		Formula/Rule: N/A
	2. Evaluate the impact of expression rate on protein folding.
		Working: If proteins are synthesized rapidly, they may not have sufficient time to fold correctly, leading to aggregation.
		Key Point: High expression rates increase the likelihood of misfolded proteins.
	3. Assess the other options and their relevance to inclusion body formation.
		Result: Other conditions like low temperatures or abundant chaperones do not typically lead to inclusion body formation, but rather help in proper folding.
		Verification: High expression rates correlate clearly with the formation of inclusion bodies in recombinant protein production.
## OPTION ANALYSIS
[A] ✓: High expression rates of recombinant proteins can overwhelm the folding machinery, leading to misfolding and the formation of inclusion bodies.
[B] :x: Low temperatures during protein synthesis generally promote proper folding and can reduce aggregation.
[C] :x: Abundant chaperone proteins assist in correct protein folding, reducing the chances of inclusion body formation.
[D] :x: Slow protein synthesis rates usually allow for proper folding and are less likely to result in inclusion bodies.
## LEARNING AIDS
	**Common Mistakes**:
		1. Confusing high expression rates with proper folding conditions: Remember that high expression can lead to misfolding.
		2. Misunderstanding the role of chaperones: They help prevent inclusion body formation, rather than cause it.
	**Quick Tips**:
		1. Recall that inclusion bodies form when there's a bottleneck in the protein folding process.
		2. The term 'inclusion bodies' indicates misfolded aggregates, which are mostly a result of rapid overexpression.

K SURESH KUMAR · Answer

The correct Option is "High expression rates of recombinant proteins"

K SURESH KUMAR · Answer

Low temperatures during protein synthesis

K SURESH KUMAR · Answer

Abundant chaperone proteins in the cell

K SURESH KUMAR · Answer

Slow protein synthesis rates

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