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    2.

    If by radiation all nitrogenase enzymes are inactivated, then there will be no

    A

    Fixation of nitrogen in legumes

    B

    Fixation of atmospheric nitrogen

    C

    Conversion from nitrate to nitrite in legumes

    D

    Conversion from ammonium to nitrate in soil

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    4.

    The free energy change (Ξ”G\Delta G) for nitrogen fixation is highly positive. Which of the following BEST explains how biological nitrogen fixation occurs despite this energetic barrier?

    A

    The reaction proceeds spontaneously at high temperatures found in root nodules

    B

    Nitrogenase acts as a catalyst, lowering the activation energy but not the Ξ”G\Delta G

    C

    Hydrolysis of a large number of ATP molecules coupled to the reaction

    D

    The presence of leghemoglobin alters the thermodynamics, making Ξ”G\Delta G negative

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    8.

    Nitrosomonas and Nitrococcus are involved in

    A

    oxidation of nitrates into nitrites

    B

    reduction of nitrates to nitrites

    C

    oxidation of ammonia into nitrites

    D

    reduction of nitrates to ammonia

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