A researcher is studying facilitated diffusion of a novel solute 'X' across a cell membrane using an artificial lipid bilayer system. They observe that increasing the concentration of X on one side of the membrane increases the rate of transport, but only up to a certain point. Beyond this point, further increases in X concentration do not affect the transport rate. Which of the following BEST explains this observation?
The membrane has reached its maximum permeability for X.
The solute X is being actively transported at higher concentrations.
Saturation of the carrier proteins
The solute X is inhibiting its own transport through feedback regulation.
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