The Question is from neet-chemistry basic-chemistry-concepts , and it was created by the author Mr. S M HUSSAIN

Question

Which of the following methods is NOT suitable for concentrating a protein solution?

Mr. S M HUSSAIN · Accepted Answer

## CORE INFORMATION
✓ **Answer**: A
## CONCEPT FRAMEWORK
 **Primary Concept**: Protein concentration techniques
 **Related Topics**:  Dialysis, ultrafiltration, lyophilization, distillation
 **Prerequisites**: Understanding of basic separation techniques and their applications to biomolecules.
## SOLUTION PATHWAY
**Given Information**:
  The question asks to identify a method unsuitable for concentrating protein solutions.
  Options include simple distillation, ultrafiltration, lyophilization, and dialysis against a concentrated polymer solution.
**Method & Steps**:
 1. Analyze each method to determine its suitability for protein concentration.
  Reasoning: We need to consider whether each method removes solvent (water) to increase protein concentration without damaging the protein.
 2. Evaluate each option:
  Simple distillation: This involves boiling the solution and collecting the vapor.  Proteins will denature (lose their structure and function) at high temperatures.
  Ultrafiltration: This uses a membrane to separate proteins from smaller molecules like water, effectively concentrating the protein solution.
  Lyophilization (freeze-drying): This involves freezing the solution and then removing the ice by sublimation.  This is a gentle method that preserves protein structure.
  Dialysis against a concentrated polymer solution: This uses a semipermeable membrane to allow water to move from the protein solution to a concentrated polymer solution, leading to protein concentration.
 3. Identify the unsuitable method:
  Result: Simple distillation is not suitable because it uses high heat which denatures proteins.
  Verification: The other options are known protein concentration methods, confirming simple distillation as the unsuitable one.
## OPTION ANALYSIS
A ✓: Simple distillation:  High temperatures used in distillation denature proteins, making it unsuitable for concentrating them. 
B :x: Ultrafiltration: This method effectively concentrates proteins by removing water and small molecules.
C :x: Lyophilization: This gentle method removes water through sublimation, preserving protein structure and concentration.
D :x: Dialysis against a concentrated polymer solution: This method uses osmotic pressure to remove water, concentrating the protein solution.
## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing distillation with other separation techniques: Understanding the principle of each method is crucial for correct selection.
  2.Overlooking the effect of temperature on proteins: High temperatures lead to protein denaturation.
 **Quick Tips**:
  1.Remember that protein concentration methods should be gentle to avoid denaturation.
  2.Consider the size and properties of the protein when choosing a concentration method.

Mr. S M HUSSAIN · Answer

The correct Option is "Simple distillation"

Mr. S M HUSSAIN · Answer

Ultrafiltration

Mr. S M HUSSAIN · Answer

Lyophilization

Mr. S M HUSSAIN · Answer

Dialysis against a concentrated polymer solution

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