The Question is from neet-botany biological-classification , and it was created by the author Mr. NARESH KUMAR K

Question

Which of the following is not characteristic of Gram positive bacteria?

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: D. Outer membrane with Lipopolysaccharide (LPS)
## CONCEPT FRAMEWORK
	**Primary Concept**: Bacterial Cell Wall Structure (Gram Staining)
	**Related Topics**: Microbiology, Bacteriology, Peptidoglycan, Teichoic Acids, Lipopolysaccharide (LPS)
	**Prerequisites**: Basic understanding of prokaryotic cell structure and the Gram staining procedure.
## SOLUTION PATHWAY
**Given Information**:
	Characteristics of Gram-positive and Gram-negative bacteria.
**Method & Steps**:
	1. **Recall Gram-positive cell wall characteristics**:
		Reasoning: Gram-positive bacteria have a distinctive cell wall structure.
	2. **Compare options with known characteristics**:
		Working: Evaluate each option against the typical features of Gram-positive bacteria.
	3. **Identify the incorrect characteristic**:
		Result: The presence of an outer membrane with LPS is a hallmark of Gram-negative bacteria, not Gram-positive.
		Verification: Gram-positive bacteria lack an outer membrane and LPS.
## OPTION ANALYSIS
[A] Thick peptidoglycan layer: ✓: Gram-positive bacteria possess a thick peptidoglycan layer, which is a key feature contributing to their Gram stain result.
[B] Purple Gram stain result: ✓: The thick peptidoglycan layer retains the crystal violet dye during the Gram stain procedure, resulting in a purple color.
[C] Teichoic acids in the cell wall: ✓: Teichoic acids are unique to Gram-positive bacteria and are embedded within the peptidoglycan layer.
[D] Outer membrane with Lipopolysaccharide (LPS): :x: An outer membrane containing LPS is a characteristic of Gram-negative bacteria.  Gram-positive bacteria lack this outer membrane.
## LEARNING AIDS
	**Common Mistakes**:
		1. Confusing the characteristics of Gram-positive and Gram-negative bacteria: Carefully review the distinct features of each type.
		2. Misinterpreting the role of the peptidoglycan layer:  Understand how the thickness of the peptidoglycan layer affects the Gram stain result.
	**Quick Tips**:
		1. Remember 'P' for Positive, Purple, and Peptidoglycan (thick).
		2. Associate LPS with the outer membrane of Gram-negative bacteria.  'L' for LPS and 'L' for the extra Layer (outer membrane).

Mr. NARESH KUMAR K · Answer

Thick peptidoglycan layer

Mr. NARESH KUMAR K · Answer

Purple Gram stain result

Mr. NARESH KUMAR K · Answer

Teichoic acids in the cell wall

Mr. NARESH KUMAR K · Answer

The correct Option is "Outer membrane with Lipopolysaccharide (LPS)"

NEET Botany Biological Classification MCQs

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