The Question is from neet-botany biomolecules-biology introduction, and it was created by the author B VENKAT

Question

Lipid are found in acid insoluble fraction during the analysis of chemical composition of tissues. Given the reason

B VENKAT · Accepted Answer

## CORE INFORMATION
✓ **Answer**: D
## CONCEPT FRAMEWORK
**Primary Concept**:  Cell Fractionation and Solubility of Biomolecules
**Related Topics**:  Lipid Structure, Biomembranes, Tissue Analysis, Centrifugation
**Prerequisites**: Basic knowledge of cell biology, biomolecules, and lab techniques.
## SOLUTION PATHWAY
**Given Information**:
- Lipids are found in the acid-insoluble fraction during tissue analysis.
- The question asks for the reason behind this observation.
**Method & Steps**:
1. **Understanding the process:** Tissue analysis often involves grinding or homogenization to break open cells. This process releases cellular components.
    Reasoning: This step is crucial because it disrupts the cell structure, allowing for the separation of different biomolecules.
2. **Analyzing lipid behavior:** Lipids are primarily found in cell membranes. These membranes are composed of lipids and proteins. When cells are broken, the membranes fragment.
    Working: Lipid molecules, being hydrophobic, tend to aggregate and form vesicles to minimize contact with the aqueous environment.
    Key Point: The key is that the membranes don't dissolve into individual molecules, but rather form insoluble vesicles.
3. **Relating to acid-insoluble fraction**: Because these lipid vesicles are not soluble in the acidic solution used in the cell fractionation process, they precipitate out and end up in the insoluble fraction.
    Result: Option D is the most accurate.
    Verification: Other options relate to molecular weight or polymeric nature, which are not the primary reasons for lipids being in the insoluble fraction.
 ## OPTION ANALYSIS
[A] :x:  While some lipids have larger molecular weights, it's not the primary reason for their presence in the insoluble fraction. Many small lipids also end up in the insoluble fraction.
[B] :x: Lipids are not typically considered true polymers like proteins or nucleic acids. This is not the reason for their insolubility.
[C] :x: Lipids, although having a lower molecular weight than many biomolecules, are insoluble because of their hydrophobic nature and membrane structure, not just their low molecular weight.
[D] ✓: This is the correct explanation. The disruption of cell membranes during grinding causes lipids to form insoluble vesicles, leading to their presence in the acid-insoluble fraction.
 ## LEARNING AIDS
**Common Mistakes**:
1.  Thinking solubility is only based on molecular weight: Many students might incorrectly assume that low molecular weight automatically means solubility and high molecular weight means insolubility. This is not always the case.
2.  Ignoring the membrane context: It's easy to overlook the fact that lipids exist mainly as part of the biomembrane and not as free molecules in the cell. This membrane structure directly affects their solubility.
**Quick Tips**:
1.  Think about the hydrophobic nature of lipids and how they aggregate in aqueous solutions.
2.  Visualize the breaking of the cell membrane into smaller fragments that are insoluble.

B VENKAT · Answer

It has very high molecular weight

B VENKAT · Answer

It is polymer

B VENKAT · Answer

It has low molecular weight

B VENKAT · Answer

The correct Option is "On grinding, the biomembranes are broken into pieces and form insoluble vesicles"

NEET Chemistry Classification of Elements and Periodicity in Properties Introduction MCQs

Select Topic

Question Tags

Question Tags

NEET Subjects

Our Bots

Company