Zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR-Cas9 are all tools used in gene editing. What is a key advantage of CRISPR-Cas9 over ZFNs and TALENs?
Higher target specificity and lower off-target effects
Easier design and implementation due to RNA-guided targeting
Ability to target multiple genes simultaneously without modification
Greater stability and longer lifespan within the target cell
Related Questions
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ?
pBR
Retrovirus
A phage
Ti plasmid
Adenosine Deaminase (ADA) deficiency can be cured by …A… and …B… but it is not fully curative. Here A and B can be
A-gene therapy, B-radiation therapy
A-bone marrow transplantation, B-enzyme replacement therapy
A-organ transplantation, B-hormone replacement therapy
A-radiation therapy, B-enzyme replacement therapy
Which gene is defective in individuals requiring gene therapy for ADA deficiency?
Insulin gene
Hemoglobin gene
Adenosine deaminase gene
Dystrophin gene
Which type of vector is used to introduce a functional ADA cDNA into lymphocytes, in gene therapy for ADA deficiency?
Plasmid
Bacteriophage
BAC
Retrovirus
Which of the following is/are correct about Adenosine Deaminase (ADA) deficiency?
I. In the absence of adenosine deaminase enzyme, purine metabolism is disturbed and T-lymphocytes fails to function
II. ADA deficiency is caused by the deletion of the gene for ADA
III. In some cases, it can be cured by bone marrow transplantation and enzyme replacement therapy. But in both approaches, the patients are not completely cured
IV. For permanent cure, genes isolated from the bone marrow cells producing ADA at early embryonic stages can be a possible cure
Which of the above statements are correct?
I, II and III
II, III and IV
I, III and IV
I, II, III and IV
Which one of the following gene is defective in patients suffering from Severe Combined Immunodeficiency Disease (SCID)?
Adenosine deaminase
Glutamate dehydrogenase
DNAase
Tyrosine oxidase
Adenosine deaminase (ADA) deficiency is treated using which of the following therapeutic approaches?
Antibiotic therapy
Hormone replacement therapy
Gene therapy
Physical therapy
Which of the following is the primary goal of gene therapy?
To enhance athletic performance
To treat or prevent disease by correcting genetic defects
To alter physical appearance
To create genetically modified organisms
Which of the following could be a permanent cure for treatment of Severe Combined Immuno Deficiency (SCID)?
Gene therapy
Bone marrow transplant
Enzyme replacement therapy
All of the above
Which gene therapy approach involves modifying cells outside the body and then reintroducing them?
In vivo gene therapy
Ex vivo gene therapy
Germline gene therapy
Somatic gene therapy
