What enzyme is used to 'cut' and 'paste' DNA in genetic engineering?
Restriction enzymes and ligase
DNA polymerase
RNA polymerase
Reverse transcriptase
Related Questions
Why is the production of human insulin using recombinant DNA technology preferred over extracting it from animals?
It is cheaper to produce
Animals produce insufficient quantities of insulin
Reduces the risk of allergic reactions and is more ethical
Bacteria produce more potent insulin
How are the A and B peptides of insulin connected in its mature form?
Disulfide bridges
Hydrogen bonds
Peptide bonds
C-peptide
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
The first human hormone produced by recombinant DNA technology is
Insulin
Melatonin
Testosterone
Progesterone
If the disulfide bridges in human insulin were broken, what would be the most likely consequence?
Enhanced insulin activity
Loss of insulin activity
No change in insulin activity
Increased insulin synthesis
Genetically engineered insulin is identical to human insulin because:
The human gene for insulin is used
Bacteria naturally produce human insulin
It is chemically synthesized
It is extracted from pig pancreas
With regard to insulin choose correct options.
a) C-Peptide is not present in mature insulin.
b) The insulin produced by rDNA technology has C-peptide.
c) The pro-insulin has C-peptide
d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below
(a), (c) and (d) only
(a) and (d) only
(b) and (d) only
(b) and (c) only
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
Which body of the Govt of India regulates GM research and safety of introducing GM organisms for public services?
GEAC
RCGM
Bio-Safety committee
ICAR
Which specific enzymatic cleavage is crucial for converting proinsulin to active insulin in the production of genetically engineered human insulin?
A. Cleavage at the N-terminus of the A-chain
B. Cleavage at the C-terminus of the B-chain
C. Removal of the C-peptide by specific endopeptidases
D. Cleavage of disulfide bonds linking A and B chains
