The Question is from neet-botany plant-growth-and-development , and it was created by the author Mr. NARESH KUMAR K

Question

In tissue culture experiments, leaf mesophyll cells are placed in a culture medium to form callus. This phenomenon is known as:

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: B. Dedifferentiation
## CONCEPT FRAMEWORK
 **Primary Concept**: Cell differentiation and dedifferentiation in plant tissue culture
 **Related Topics**: Plant cell totipotency, callus formation, morphogenesis
 **Prerequisites**: Basic understanding of plant cell structure and function, tissue culture techniques
## SOLUTION PATHWAY
**Given Information**:
  Leaf mesophyll cells are placed in a culture medium.
  Callus formation occurs.
**Method & Steps**:
 1. Understand the process of dedifferentiation.
  Reasoning: Dedifferentiation is the process by which differentiated cells revert to an undifferentiated state, losing their specialized characteristics.
  Formula/Rule:  N/A
 2. Analyze the given scenario.
  Working: Leaf mesophyll cells are specialized cells with specific functions. When placed in a culture medium, they lose their specialized features and become undifferentiated cells, forming a mass of cells called callus.
  Key Point: Callus formation is a hallmark of dedifferentiation in plant tissue culture.
 3. Identify the correct term.
  Result: The process described is dedifferentiation.
  Verification: The definition of dedifferentiation accurately describes the observed phenomenon.
 ## OPTION ANALYSIS
A :x: Redifferentiation is the process by which undifferentiated cells become specialized cells. This is the opposite of what's happening.
B ✓: Dedifferentiation accurately describes the reversion of specialized mesophyll cells to an undifferentiated state.
C :x: Differentiation is the process of cells becoming specialized, which is not occurring in this case.
D :x: Transdifferentiation is the change of one differentiated cell type to another differentiated cell type; this is not the case here, as the cells are becoming undifferentiated.
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing dedifferentiation with differentiation: Remember that dedifferentiation is the loss of specialized characteristics, while differentiation is the gain of specialized characteristics.
  2.Misunderstanding the role of the culture medium: The culture medium provides the necessary nutrients and hormones for the cells to dedifferentiate.
 **Quick Tips**:
  1.Think of dedifferentiation as a reversal of differentiation.
  2.Remember that callus formation is a key indicator of dedifferentiation in plant tissue culture.

Mr. NARESH KUMAR K · Answer

Redifferentiation

Mr. NARESH KUMAR K · Answer

The correct Option is "Dedifferentiation"

Mr. NARESH KUMAR K · Answer

Differentiation

Mr. NARESH KUMAR K · Answer

Transdifferentiation

NEET Botany Plant Growth and Development MCQs

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