In the context of producing genetically engineered human insulin, why is the formation of inclusion bodies in E. coli sometimes considered advantageous despite requiring additional processing steps?
A. Inclusion bodies protect insulin from proteolytic degradation within the bacterial cell
B. Inclusion bodies directly facilitate the formation of correct disulfide bonds
C. Inclusion bodies enhance the solubility and secretion of insulin from the bacterial cell
D. Inclusion bodies simplify the purification process by eliminating the need for chromatography
Related Questions
Mature insulin consists of?
Two long polypeptide chains
Two long polynucleotide chains
Two short polypeptide chains
Two short polynucleotide chains
Genetically engineered human insulin is produced by:
Bacteria
Viruses
Fungi
Plants
Which of these is NOT an advantage of using genetically engineered insulin?
Higher cost compared to animal-derived insulin
Reduced risk of allergic reactions
Larger and more reliable supply
Higher purity
Which one of the following is the most suitable, medium for culture of Drosophila melanogaster?
Moist bread
Agar agar
Ripe banana
Cow dung
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
The production of human insulin in bacteria involves the insertion of the human gene for insulin into a bacterial plasmid. This plasmid then acts as a:
Vector
Promoter
Operator
Repressor
Regarding insulin, which of the following statements are correct?
(a) Mature insulin lacks C-peptide.
(b) Insulin produced via rDNA technology contains C-peptide.
(c) Pro-insulin has C-peptide.
(d) Disulfide bridges link the A-peptide and B-peptide of insulin.
(a), (b), (c)
(a), (c), (d)
(b), (c), (d)
(a), (b), (d)
If crossing over did NOT occur during meiosis, what would be the most likely consequence?
Increased genetic variation in offspring
No change in genetic variation
Faster cell division
Reduced genetic variation in offspring
Identify the substances having glycosidic bond and peptide bond, respectively in their structure
Chitin, cholesterol
Glycerol, trypsin
Cellulose, lecithin
Inulin, insulin
A researcher is attempting to express human proinsulin in a eukaryotic expression system like yeast. Compared to bacterial systems, what distinct advantage does this offer concerning post-translational modifications?
A. Yeast can directly cleave proinsulin into active insulin without the need for enzymatic treatment
B. Yeast can perform glycosylation, which may enhance insulin stability or activity
C. Yeast prevents the formation of inclusion bodies, simplifying purification
D. Yeast systems eliminate the risk of endotoxin contamination
