The 'histone code' hypothesis proposes that specific combinations of histone modifications influence gene expression. If a researcher discovered a novel histone modification, which experimental approach would be LEAST informative for initially investigating its potential role in gene regulation?
Analyzing the genome-wide distribution of the modification using ChIP-seq.
Correlating the presence of the modification with gene expression levels.
Assessing the binding of specific regulatory proteins to the modified histone.
Determining the crystal structure of the modified histone.
Related Questions
A researcher isolates chromatin fibers and treats them with a nuclease that specifically cleaves linker DNA. Following this treatment, what would be the approximate size distribution of the DNA fragments observed?
Approximately 200 bp
Approximately 1000 bp
Approximately 50 bp
A wide range of sizes from 200 bp to several thousand bp
The presence of histone H1 in association with a nucleosome suggests:
Further compaction of chromatin structure
Transcription activation
DNA replication initiation
Nucleosome disassembly
Which of the following processes is facilitated by the binding of histone H1 to a nucleosome?
DNA unwinding
Chromatin condensation
RNA splicing
Protein synthesis
Which of the following is the basic repeating unit of chromatin?
Gene
Chromosome
Centromere
Nucleosome
The association of histone H1 with a nucleosome indicates:
The DNA double helix is exposed
Transcription is occurring
DNA replication is occurring
The DNA is condensed into a Chromatin Fibre
Approximately how many base pairs of DNA are wrapped around a histone octamer in a nucleosome?
20
146
200
1000
Approximately how many base pairs of DNA are wrapped around a single nucleosome core?
200
50
146
250
Histone H1 plays a crucial role in the structure of chromatin. Which of the following statements regarding its interaction and function is INCORRECT?
H1 binds to the linker DNA between nucleosomes.
H1 helps to compact chromatin structure.
H1 interacts with the core histones within the nucleosome octamer.
H1 stabilizes the interaction of DNA with the nucleosome.
The structure formed by DNA wrapped around histone proteins is called:
Chromosome
Chromatid
Chromatin
Centromere
Which of these processes is directly influenced by the level of chromatin compaction?
DNA replication
RNA processing
Gene expression
Protein translation
