In DNA fingerprinting, the process of separating DNA fragments of different sizes is called:
Polymerase Chain Reaction
Electrophoresis
Southern Blotting
Hybridization
Related Questions
DNA fingerprinting technique was first developed by
Jeffreys, Wilson and Thien
Boysen and Jensen
Schleiden and Schwann
Edward and Steptoe
Automated DNA sequencers, work on the principle of the method developed by
Erwin Chargaff
Maurice Wilkins
Frederick Sanger
Francis Crick
Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only little DNA could be extracted from it. If the genes of the ancient man need to be analysed, the best way of getting sufficient amount of DNA from this extract is
By hybridizing the DNA with a DNA probe
By subjecting the DNA to polymerase chain reaction
By subjecting the DNA to gel electrophoresis
By treating the DNA with restriction endonucleases
DNA fingerprinting technique was discovered by
Wilmut
A Jeffreys
Ethoven
Kary Mullis
Minisatellite or Variable Number Tendem Repeat (VNTR) are used in
Gene therapy
Gene mapping
DNA fingerprinting
Restriction enzymes
In the polymerase chain reaction (PCR), a high temperature is crucial at the beginning. If this temperature is not reached, which of the following events will be directly and primarily compromised?
DNA strand separation
Primer annealing
DNA polymerase activity
Nucleotide addition
A researcher is analyzing DNA fragments using gel electrophoresis. The gel is submerged in a buffer solution and a current is applied. After the separation, the researcher adds a solution to the gel and views it under UV light. What is the purpose of the added solution?
To provide conductivity to the gel
To denature the DNA fragments
To stain the DNA fragments for visualization
To apply an electric current to the gel
In DNA fingerprinting, which technique is NOT directly involved in generating the unique DNA profile?
Restriction digestion
Gel electrophoresis
Southern blotting
Polymerase Chain Reaction (PCR)
The unique DNA profile generated in DNA fingerprinting is based on the polymorphic nature of:
Promoters
Operators
Structural genes
VNTRs
In the context of forensic science, DNA fingerprinting utilizes variations in:
Telomeres
Centromeres
VNTRs
Histones