The Question is from neet-botany molecular-basis-of-inheritance , and it was created by the author Mr. NARESH KUMAR K

Question

During DNA replication, the enzyme responsible for removing RNA primers and replacing them with DNA is:

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: D
## CONCEPT FRAMEWORK
 **Primary Concept**: DNA Replication Enzymes
 **Related Topics**: DNA replication, RNA primers, DNA polymerase, exonuclease activity
 **Prerequisites**: Understanding of the basic steps of DNA replication.
## SOLUTION PATHWAY
**Given Information**:
The question asks about the enzyme responsible for removing RNA primers and replacing them with DNA during DNA replication.
**Method & Steps**:
1. Identify the enzymes involved in DNA replication.
  Reasoning: Several enzymes are crucial for DNA replication, including DNA polymerase I, DNA polymerase III, primase, and DNA ligase. Each enzyme has a specific function.
  Formula/Rule: N/A
2. Determine the function of each enzyme.
  Working: 
		* DNA polymerase III is the main enzyme responsible for synthesizing new DNA strands. 
		* DNA ligase joins Okazaki fragments on the lagging strand.
		* Primase synthesizes RNA primers, which provide a starting point for DNA polymerase.
		* DNA polymerase I has 5' to 3' exonuclease activity, allowing it to remove RNA primers. It also has 5' to 3' polymerase activity to replace the removed RNA with DNA.
  Key Point: The removal of RNA primers and their replacement with DNA is a crucial step in DNA replication to ensure the integrity of the newly synthesized DNA strand.
3. Identify the enzyme responsible for removing RNA primers and replacing them with DNA.
  Result: DNA polymerase I
  Verification: DNA polymerase I's 5' to 3' exonuclease activity allows it to remove the RNA primers, and its 5' to 3' polymerase activity allows it to fill in the gaps with DNA.
 ## OPTION ANALYSIS
A :x: DNA polymerase III is the main enzyme for DNA synthesis, but it doesn't remove RNA primers.
B :x: DNA ligase joins Okazaki fragments, but it doesn't remove primers.
C :x: Primase synthesizes RNA primers; it doesn't remove them.
D ✓: DNA polymerase I removes RNA primers and replaces them with DNA.
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing the roles of DNA polymerase I and DNA polymerase III: Remember that DNA polymerase III is the main replication enzyme, while DNA polymerase I is involved in primer removal and replacement.
  2.Forgetting the exonuclease activity of DNA polymerase I:  The exonuclease activity is crucial for the primer removal function.
 **Quick Tips**:
  1.Remember the key functions of each enzyme involved in DNA replication.
  2.Associate DNA polymerase I with both polymerase and exonuclease activities.

Mr. NARESH KUMAR K · Answer

DNA polymerase III

Mr. NARESH KUMAR K · Answer

DNA ligase

Mr. NARESH KUMAR K · Answer

Primase

Mr. NARESH KUMAR K · Answer

The correct Option is "DNA polymerase I"

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