During DNA replication, topoisomerases play a crucial role. Which statement BEST describes the function of topoisomerases?
They synthesize RNA primers for DNA polymerase.
They unwind the DNA double helix at the replication fork.
They relieve torsional stress in the DNA helix ahead of the replication fork.
They join Okazaki fragments on the lagging strand.
Related Questions
What is the role of DNA ligase in DNA replication?
Unwinds DNA
Synthesizes RNA primers
Joins Okazaki fragments
Adds nucleotides to the growing DNA strand
In E. coli, DNA replication initiates at a specific site called the:
Promoter
Terminator
Origin of replication
Operator
DNA strand which is formed continuously in 5’ 3’ direction is called
Lagging strand
Leading strand
Template strand
Stranded strand
Okazaki fragments are joined in a correct sequence by
DNA polymerase
DNA ligase
RNA polymerase
Primase
Which of these acts as a starting point for DNA synthesis by DNA polymerase?
Okazaki fragment
DNA template
RNA primer
Helicase
The enzyme used to join the DNA fragments is
Topoisomerase
Adenosine deaminase
DNA ligase
DNA polymerase
The enzyme which helps to cut one strand of DNA duplex to release tension of coiling of two strands is
DNA ligase
DNA polymerase-I
Topoisomerase
Swielases (helicase or unwindases)
The RNA primer is used in
Translation
Replication
Conjugation
Transformation
Meselson and Stahl experiment on semi-conservative replication demonstrates
50% radioactive, 50% non-radioactive
50% non-radioactive
50% radioactive
None of the above
Okazaki fragments are joined in a correct sequence by
DNA polymerase
DNA ligase
RNA polymerase
Primase
