The Question is from neet-botany biotechnology biotechnological-applications-in-agriculture, and it was created by the author D VINOD

Question

Which gene transfer method is commonly used to create transgenic plants?

D VINOD · Accepted Answer

## CORE INFORMATION
✓ **Answer**: C
## CONCEPT FRAMEWORK
 **Primary Concept**: Transgenic plant creation methods
 **Related Topics**: Genetic engineering, plant biotechnology, gene expression
 **Prerequisites**: Basic understanding of gene transfer techniques and plant biology.
## SOLUTION PATHWAY
**Given Information**:
  The question asks about the commonly used gene transfer method for creating transgenic plants.
  Options: Electroporation, Microinjection, Agrobacterium-mediated transformation, Particle bombardment.
**Method & Steps**:
 1. Analyze each option's application in plant genetic engineering.
  Reasoning: We need to identify the most widely used and effective method for introducing foreign genes into plant cells.
  Formula/Rule: None directly applicable; this is a knowledge-based question.
 2. Evaluate the efficiency and practicality of each method for plant transformation.
  Working: 
            * **Electroporation:** Creates transient pores in cell membranes to allow DNA entry, but efficiency is often lower in plants.
            * **Microinjection:** Directly injecting DNA into plant cells, but technically challenging and low throughput.
            * **Agrobacterium-mediated transformation:** Uses the natural ability of *Agrobacterium tumefaciens* to transfer DNA into plant cells; highly efficient and widely used.
            * **Particle bombardment (biolistics):** Shoots DNA-coated particles into plant cells; useful for recalcitrant species but can cause damage.
  Key Point:  The choice of method depends on the plant species and the desired outcome, but *Agrobacterium* is the most common and generally preferred method due to its high efficiency and relatively simple procedure.
 3. Determine the most commonly used method.
  Result: Agrobacterium-mediated transformation (option C)
  Verification:  Extensive literature and practical experience in plant biotechnology support the widespread use of *Agrobacterium* for transgenic plant creation.
 ## OPTION ANALYSIS
A :x: Electroporation is less efficient than Agrobacterium-mediated transformation in plants.
B :x: Microinjection is technically difficult and less efficient for plant transformation.
C ✓: Agrobacterium-mediated transformation is a highly efficient and widely used method for creating transgenic plants.
D :x: Particle bombardment is used, but less frequently than Agrobacterium-mediated transformation, especially for dicots.
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing the efficiency and applicability of different gene transfer methods in plants:  Knowing the strengths and weaknesses of each method is crucial.
  2. Overlooking the widespread use of Agrobacterium-mediated transformation in favor of less efficient techniques.
 **Quick Tips**:
  1. Remember that Agrobacterium is a natural vector for gene transfer in plants.
  2.  Consider the relative efficiency, cost, and ease of use when comparing gene transfer methods for plant transformation.

D VINOD · Answer

Electroporation

D VINOD · Answer

Microinjection

D VINOD · Answer

The correct Option is "Agrobacterium-mediated transformation"

D VINOD · Answer

Particle bombardment

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