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NEET Questions / Botany / Biotechnology and Its Applications / Biotechnological Applications In Agriculture
A scientist is attempting to develop a transgenic crop resistant to a specific lepidopteran pest. Which gene would be the LEAST suitable candidate for transfer?
A gene encoding a Bt toxin
A gene conferring resistance to a bacterial pathogen
A gene involved in photosynthesis
A gene encoding a protein that disrupts insect gut function
Which combination of technologies is MOST likely to be employed in developing a 'Golden Rice' variety with enhanced iron bioavailability?
Phytoene synthase gene transfer, Lycopene beta-cyclase gene transfer
Ferritin gene transfer, Phytase gene transfer
Cry gene transfer, Proteinase inhibitor gene transfer
Viral coat protein gene transfer, Chitinase gene transfer
A researcher uses Agrobacterium tumefaciens to introduce a gene into a plant cell. Which of the following is NOT a crucial step in this process?
Infection of plant cells with the engineered Agrobacterium
Insertion of the gene of interest into the Ti plasmid
Ligation of the gene into a yeast expression vector
Regeneration of the transformed plant cells
Which technique is LEAST suitable for introducing a foreign gene into a mature plant cell with a thick cell wall?
Biolistics
Agrobacterium-mediated transformation
Microinjection
Heat shock transformation
RNA interference (RNAi) technology is being explored for developing pest-resistant plants. Which statement about RNAi is INCORRECT?
The RNAi effect can be highly specific to the target pest.
The introduced dsRNA is processed into smaller interfering RNAs (siRNAs).
RNAi primarily works by blocking translation of target mRNA.
The RNAi trait is easily inherited through traditional plant breeding methods.
A new herbicide-tolerant crop variety has been developed. Which evaluation is MOST crucial before its commercial release, considering potential environmental impact?
Analysis of the nutritional content of the modified crop
Evaluation of the crop's yield compared to traditional varieties
Assessment of gene flow to wild relatives and the development of herbicide resistance in weeds
Study of the effects of the herbicide on non-target insects
Which of the following is NOT a benefit of using genetically modified (GM) crops in agriculture?
Enhanced nutritional value
Increased crop yield
Increased susceptibility to pests
Reduced reliance on pesticides
Bt cotton is a genetically modified crop that produces a toxin derived from:
Agrobacterium tumefaciens
Bacillus thuringiensis
Escherichia coli
Saccharomyces cerevisiae
Golden rice is a genetically modified variety designed to enhance the production of:
Vitamin C
Vitamin A
Iron
Protein
Which gene transfer method is commonly used to create transgenic plants?
Electroporation
Microinjection
Agrobacterium-mediated transformation
Particle bombardment