During the production of genetically engineered human insulin, which of the following is NOT a critical step in ensuring the correct formation of disulfide bonds essential for the protein's biological activity?
Correct protein folding
Oxidation of cysteine residues
Cleavage of the proinsulin molecule
Glycosylation of the protein
Related Questions
Which of the following peptide chain is removed during the maturation of proinsulin into insulin?
A-chain (21 amino acid)
B-chain (30 amino acid)
C-chain (33 amino acid)
A and B chain
What enzyme is used to 'cut' and 'paste' DNA in genetic engineering?
Restriction enzymes and ligase
DNA polymerase
RNA polymerase
Reverse transcriptase
Which specific enzymatic cleavage is crucial for converting proinsulin to active insulin in the production of genetically engineered human insulin?
A. Cleavage at the N-terminus of the A-chain
B. Cleavage at the C-terminus of the B-chain
C. Removal of the C-peptide by specific endopeptidases
D. Cleavage of disulfide bonds linking A and B chains
First hormone produced by recombinant DNA technology is
Growth hormone
Insulin
Estrogen
Erythropoietin
Apart from disulfide bridges, which other interactions contribute to the overall three-dimensional structure of insulin?
Peptide bonds only
Glycosidic bonds and phosphodiester bonds
Hydrogen bonds, hydrophobic interactions, and van der Waals forces
Ionic bonds only
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
Insulin used for diabetes was earlier extracted from?
Liver of slaughtered cattle and pigs
Lungs of slaughtered cattle and pigs
Pancreas of slaughtered cattle and pigs
Pancreas of mice and pigs.
Which enzyme is crucial for joining the insulin gene with the plasmid vector?
DNA ligase
RNA polymerase
Reverse transcriptase
Restriction endonuclease
The first human hormone produced by recombinant DNA technology is
Insulin
Melatonin
Testosterone
Progesterone
Which two chains make up the mature, functional human insulin molecule produced by genetic engineering?
A and B chains linked by disulfide bonds
A and C chains linked by hydrogen bonds
B and C chains linked by peptide bonds
Alpha and beta chains linked by glycosidic bonds
