Which two chains make up the mature, functional human insulin molecule produced by genetic engineering?
A and B chains linked by disulfide bonds
A and C chains linked by hydrogen bonds
B and C chains linked by peptide bonds
Alpha and beta chains linked by glycosidic bonds
Related Questions
Crossing over, a crucial process for genetic diversity, occurs during which stage of meiosis?
Prophase II
Metaphase I
Anaphase I
Prophase I
Which body of the Govt of India regulates GM research and safety of introducing GM organisms for public services?
GEAC
RCGM
Bio-Safety committee
ICAR
Which one of the following is the most suitable, medium for culture of Drosophila melanogaster?
Moist bread
Agar agar
Ripe banana
Cow dung
Why is genetically engineered insulin preferred over insulin derived from animal sources?
It reduces the risk of allergic reactions and is more readily available.
It is less effective than animal insulin.
It is more expensive to produce.
It is not as pure as animal insulin.
In genetically engineered insulin production, what is the role of ฮฒ-galactosidase?
Selection marker for transformed bacteria
Cleaves proinsulin into mature insulin
Promotes insulin gene transcription
Facilitates the uptake of the plasmid
What enzyme is used to 'cut' and 'paste' DNA in genetic engineering?
Restriction enzymes and ligase
DNA polymerase
RNA polymerase
Reverse transcriptase
Which type of bond is crucial for maintaining the structural integrity of the two polypeptide chains in human insulin?
Hydrogen bond
Disulfide bond
Glycosidic bond
Phosphodiester bond
Consider the following statements about insulin
I. Human insulin is made-up of 51 amino acids arranged in two polypeptide chains
II. The two polypeptide chains are interconnected by two disulphide bridges
III. In mammals including humans, insulin is synthesized as a pro-hormone, which contains an extra stretch called the C-peptide
IV. C-peptide is not present in the mature insulin
Which of the statements given above are correct?
I, II and III
I, III and IV
II, III and IV
I, II, III and IV
In the context of producing genetically engineered human insulin, why is the formation of inclusion bodies in E. coli sometimes considered advantageous despite requiring additional processing steps?
A. Inclusion bodies protect insulin from proteolytic degradation within the bacterial cell
B. Inclusion bodies directly facilitate the formation of correct disulfide bonds
C. Inclusion bodies enhance the solubility and secretion of insulin from the bacterial cell
D. Inclusion bodies simplify the purification process by eliminating the need for chromatography
Which one of the following is the most suitable, medium for culture of Drosophila melanogaster?
Moist bread
Agar agar
Ripe banana
Cow dung
