What is a primary concern regarding the immunogenicity of genetically engineered insulin produced in bacterial systems, and how is this typically addressed?
A. The altered amino acid sequence of engineered insulin is highly immunogenic; humanized antibodies are co-administered.
B. The use of antibiotic resistance genes in production vectors triggers immune reactions; marker-free vectors are used.
C. The lack of glycosylation in bacterially produced insulin increases its immunogenicity; glycosylation enzymes are added in vitro.
D. Bacterial contaminants like endotoxins can elicit immune responses; purification methods are employed to remove them.
Related Questions
The production of human insulin in bacteria involves the insertion of the human gene for insulin into a bacterial plasmid. This plasmid then acts as a:
Vector
Promoter
Operator
Repressor
In genetically engineered insulin production, what is the role of ฮฒ-galactosidase?
Selection marker for transformed bacteria
Cleaves proinsulin into mature insulin
Promotes insulin gene transcription
Facilitates the uptake of the plasmid
Why is genetically engineered insulin preferred over insulin derived from animal sources?
It reduces the risk of allergic reactions and is more readily available.
It is less effective than animal insulin.
It is more expensive to produce.
It is not as pure as animal insulin.
Identify the substances having glycosidic bond and peptide bond, respectively in their structure
Chitin, cholesterol
Glycerol, trypsin
Cellulose, lecithin
Inulin, insulin
Which body of the Govt of India regulates GM research and safety of introducing GM organisms for public services?
GEAC
RCGM
Bio-Safety committee
ICAR
Which statement incorrectly describes the role of cell division in organisms?
Meiosis is crucial for sexual reproduction.
Mitosis is primarily involved in growth and repair.
Both mitosis and meiosis involve DNA replication.
Mitosis is the process of gamete formation.
Which type of bond is crucial for maintaining the structural integrity of the two polypeptide chains in human insulin?
Hydrogen bond
Disulfide bond
Glycosidic bond
Phosphodiester bond
Which of these describes the monomers joined by a glycosidic bond and a peptide bond, respectively?
A) Monosaccharides, Amino acids
B) Monosaccharides, Nucleotides
C) Fatty acids, Glucose
D) Amino acids, Disaccharides
Mature insulin consists of?
Two long polypeptide chains
Two long polynucleotide chains
Two short polypeptide chains
Two short polynucleotide chains
