The Question is from neet-botany biotechnology genetically-engineered-insulin, and it was created by the author B VENKAT

Question

The gene for human insulin is inserted into the bacteria's:

B VENKAT · Accepted Answer

## CORE INFORMATION
✓ **Answer**: A
## CONCEPT FRAMEWORK
 **Primary Concept**: Genetic Engineering and Recombinant DNA Technology
 **Related Topics**: Plasmids, bacterial transformation, gene expression, insulin production
 **Prerequisites**: Basic understanding of DNA, genes, plasmids, and bacterial cell structure.
## SOLUTION PATHWAY
**Given Information**:
  The question asks where the human insulin gene is inserted in bacteria for insulin production.
  Bacteria are used as a host for producing human insulin.
**Method & Steps**:
 1. Identify the bacterial component used for gene insertion.
  Reasoning:  To express a foreign gene (like the human insulin gene) in bacteria, a vector is needed to carry and deliver the gene into the bacterial cell.  Plasmids are commonly used vectors in genetic engineering.
  Formula/Rule: None directly applicable, this is a conceptual understanding question.
 2. Eliminate incorrect options.
  Working:  Chromosomes are the main bacterial genetic material; inserting into the chromosome is more complex than using a plasmid. Ribosomes are involved in protein synthesis, not gene insertion. The cell wall provides structural support, not a location for gene insertion.
  Key Point: Plasmids are small, circular DNA molecules that replicate independently of the bacterial chromosome.  They are easily manipulated and used to introduce foreign genes into bacteria.
 3. Confirm the correct option.
  Result: Plasmid is the correct answer.
  Verification:  The process of inserting a gene into a plasmid is a key step in creating genetically modified organisms (GMOs) for various applications, including pharmaceutical production like insulin.
 ## OPTION ANALYSIS
A ✓: Plasmid: Correct. Plasmids are extrachromosomal DNA molecules in bacteria, often used as vectors to introduce foreign genes for expression.
B :x: Chromosome: Incorrect. While possible, inserting a gene directly into the bacterial chromosome is more complex and less efficient than using a plasmid.
C :x: Ribosome: Incorrect. Ribosomes are cellular machinery for protein synthesis, not a location for gene insertion.
D :x: Cell wall: Incorrect. The cell wall is a structural component of the bacteria, not involved in gene expression.
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing plasmids with chromosomes: Understanding the difference between these two types of DNA is essential.
  2.Thinking ribosomes are involved in gene insertion: Ribosomes are involved in protein translation, not gene insertion.
 **Quick Tips**:
  1.Remember that plasmids are used as vectors in genetic engineering to carry foreign genes into bacteria.
  2.Visualize the structure of a bacterial cell to understand where the different components are located.

B VENKAT · Answer

The correct Option is "Plasmid"

B VENKAT · Answer

Chromosome

B VENKAT · Answer

Ribosome

B VENKAT · Answer

Cell wall

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