The Question is from neet-botany biotechnology transgenic-animals, and it was created by the author Mr. NARESH KUMAR K

Question

Which technique is NOT typically used for the production of transgenic animals?

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: 3
## CONCEPT FRAMEWORK
 **Primary Concept**: Transgenic animal production techniques
 **Related Topics**: Genetic engineering, molecular biology, developmental biology
 **Prerequisites**: Understanding of fertilization, embryonic development, gene transfer methods.
## SOLUTION PATHWAY
**Given Information**:
  The question asks to identify a technique NOT used in creating transgenic animals.
  Options: Microinjection, retroviral infection, electroporation, embryonic stem cell transfer.
**Method & Steps**:
 1. Analyze each option to determine its typical use in transgenic animal production.
  Reasoning: We need to evaluate if each technique is a standard method for generating transgenic animals.
  Formula/Rule: None directly applicable.
 2. Identify the technique that is not commonly employed for creating transgenic animals.
  Working:  Let's examine each option:
  * **Microinjection:** Directly injecting DNA into a fertilized egg's pronucleus is a common method.
  * **Retroviral infection:**  Retroviruses can be used to deliver genes into embryonic stem cells, which can then be used to create transgenic animals.
  * **Electroporation:**  While used in some genetic engineering contexts, it's less common than microinjection for creating transgenic animals, especially compared to the other options.
  * **Embryonic stem cell transfer:**  Introducing genetically modified embryonic stem cells into a blastocyst is a standard approach for generating transgenic animals.
  Key Point: Electroporation is less efficient and less commonly used than the other methods for generating transgenic animals, especially when compared to the directness of pronuclear microinjection.
 3. Determine the least commonly used technique.
  Result: Electroporation (option C) is not typically the primary method used for producing transgenic animals compared to the others.
  Verification:  The other options are all established and widely used methods in transgenic animal production.  Electroporation, while possible, is less efficient and prevalent.
 ## OPTION ANALYSIS
A ✓: Microinjection of DNA into the pronucleus of a fertilized egg is a common technique.
B :x: Retroviral infection of embryonic stem cells is a frequently used method.
C :x: Electroporation of fertilized eggs with recombinant DNA is less common and less efficient than other methods.
D :x: Transfer of genetically modified embryonic stem cells into a blastocyst is a standard technique.
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing electroporation with other gene transfer methods:  Understanding the specific applications of each technique is crucial.
   How to avoid: Thoroughly learn the mechanisms and applications of each gene transfer method.
  2.Overlooking the relative efficiency of different techniques:  Some methods are simply more widely used due to their higher success rates.
   How to avoid:  Focus on the practical applications and efficiency of each method in the context of transgenic animal production.
 **Quick Tips**:
  1.Remember the primary methods: Microinjection and embryonic stem cell transfer are the most common.
  2.Consider the efficiency and practicality:  The less efficient methods tend to be less frequently used.

Mr. NARESH KUMAR K · Answer

Microinjection of DNA into the pronucleus of a fertilized egg

Mr. NARESH KUMAR K · Answer

Retroviral infection of embryonic stem cells

Mr. NARESH KUMAR K · Answer

The correct Option is "Electroporation of fertilized eggs with recombinant DNA"

Mr. NARESH KUMAR K · Answer

Transfer of genetically modified embryonic stem cells into a blastocyst

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