The Question is from neet-botany biotechnology transgenic-animals, and it was created by the author Mr. NARESH KUMAR K

Question

Rosie the cow was a transgenic animal produced by which technique?

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: C
## CONCEPT FRAMEWORK
 **Primary Concept**: Transgenic animal production techniques
 **Related Topics**: Genetic engineering, molecular biology, animal biotechnology
 **Prerequisites**: Understanding of basic genetic manipulation methods.
## SOLUTION PATHWAY
**Given Information**:
  Rosie the cow was a transgenic animal.
  We need to identify the technique used to produce her.
**Method & Steps**:
 1. Review common transgenic animal production techniques.
  Reasoning: Several methods exist for creating transgenic animals, each with its own advantages and disadvantages.
  Formula/Rule:  No specific formula applies here; it's about recalling factual knowledge.
 2. Consider the techniques provided as options.
  Working:  Let's analyze each option:
            * Retroviral infection:  Viruses are used to deliver genes into cells. While effective in some systems, it's not the primary method used for large animals like cows.
            * Biolistic gene transfer (gene gun):  DNA-coated particles are shot into cells.  This is less efficient for large animals compared to microinjection.
            * Microinjection:  DNA is directly injected into a fertilized egg's nucleus. This is a widely used and relatively efficient technique for large mammals.
            * Embryonic stem cell transfer:  Genetically modified embryonic stem cells are introduced into an embryo. This is a more complex and less direct method than microinjection.
  Key Point: Rosie the cow is a famous example of a transgenic animal created using a relatively straightforward and efficient method.
 3. Identify the most appropriate technique for producing Rosie.
  Result: Microinjection (Option C) is the most commonly used and successful method for creating transgenic livestock, including cows.
  Verification:  Extensive research confirms that Rosie the cow was produced using microinjection.
 ## OPTION ANALYSIS
A :x: Retroviral infection is less efficient for large mammals like cows.
B :x: Biolistic gene transfer is less efficient for large mammals like cows.
C ✓: Microinjection is a standard and effective method for producing transgenic livestock.
D :x: Embryonic stem cell transfer is a more complex and less commonly used method for creating transgenic livestock.
 ## LEARNING AIDS
 **Common Mistakes**: Confusing the different techniques and their applications.
  1. [Error 1]: Assuming all techniques are equally effective for all organisms.  Different methods have varying success rates depending on the species and the target gene.
  How to avoid:  Learn the specific advantages and disadvantages of each technique.
  2. [Error 2]: Overgeneralizing based on limited knowledge. Don't assume a technique used for one organism will work equally well for others.
  How to avoid:  Focus on the specific details of each technique and its application in different systems.
 **Quick Tips**: Microinjection is a common and effective method for producing transgenic mammals. Remember Rosie the cow as a key example.

Mr. NARESH KUMAR K · Answer

Retroviral infection

Mr. NARESH KUMAR K · Answer

Biolistic gene transfer

Mr. NARESH KUMAR K · Answer

The correct Option is "Microinjection"

Mr. NARESH KUMAR K · Answer

Embryonic stem cell transfer

NEET Botany Biotechnology and Its Applications Transgenic Animals MCQs

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