The Question is from neet-botany , and it was created by the author Mr. NARESH KUMAR K

Question

Grinding of a living tissue in trichloroacetic acid shows the presence of the inorganic compounds like sulphate, phosphate etc, which are categorised in

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: B
## CONCEPT FRAMEWORK
**Primary Concept**: Acid Solubility of Biomolecules
**Related Topics**: Cell Fractionation, Biochemical Composition of Cells, Inorganic and Organic Compounds
**Prerequisites**: Basic knowledge of cell structure and biomolecule classification
## SOLUTION PATHWAY
**Given Information**:
 - Living tissue is ground in trichloroacetic acid (TCA).
 - Inorganic compounds like sulphate and phosphate are observed.
**Method & Steps**:
 1. **Understanding Acid Solubility**: TCA is a strong acid used to precipitate macromolecules (like proteins and nucleic acids) which become insoluble in acidic medium.
    Reasoning: The question tests the understanding of how TCA separates different cellular components based on their solubility.
  2.  **Identifying Inorganic Compounds**: Inorganic compounds like sulfates and phosphates are small molecules.
    Reasoning: Small molecules are generally soluble in aqueous solutions.
 3. **Determining Solubility in TCA**: Since TCA precipitates macromolecules, small inorganic compounds remain dissolved in the acidic solution.
    Reasoning: The acid-soluble fraction contains the small molecules that don't precipitate.
    
**Result**: The correct answer is the acid-soluble fraction.
**Verification**: The acid soluble fraction is defined by the components that remain dissolved in the acidic solution, which includes small inorganic molecules like sulfates and phosphates.
## OPTION ANALYSIS
[A] :x: Acid insoluble fraction would contain the precipitated macromolecules, not the inorganic salts.
[B] ✓: Acid soluble fraction contains the smaller, soluble molecules, including the inorganic compounds mentioned.
[C] :x: Both fractions cannot contain the same inorganic compounds; they are separated by acid solubility.
[D] :x: The inorganic compounds are part of the cellular pool and are not absent.
## LEARNING AIDS
**Common Mistakes**:
 1. Confusing acid-soluble and acid-insoluble fractions. Understand the difference in behavior of macromolecules and small molecules in acidic conditions.
 2.  Not remembering that inorganic compounds are typically small and soluble in aqueous solutions.
**Quick Tips**:
 1. Remember that acids precipitate large molecules like proteins and nucleic acids, leaving smaller molecules in solution.
 2. Inorganic ions are generally small and thus remain soluble.

Mr. NARESH KUMAR K · Answer

Acid insoluble fraction

Mr. NARESH KUMAR K · Answer

The correct Option is "Acid soluble fraction"

Mr. NARESH KUMAR K · Answer

Both (a) and (b)

Mr. NARESH KUMAR K · Answer

Not found in cellular pool

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