The Question is from neet-botany biotechnology genetically-engineered-insulin, and it was created by the author D VINOD

Question

What enzyme is used to 'cut' and 'paste' DNA in genetic engineering?

D VINOD · Accepted Answer

## CORE INFORMATION
✓ **Answer**: A
## CONCEPT FRAMEWORK
 **Primary Concept**: Genetic engineering enzymes
 **Related Topics**: Molecular biology, recombinant DNA technology
 **Prerequisites**: Basic understanding of DNA structure and function
## SOLUTION PATHWAY
**Given Information**:
  The question asks about the enzymes used to cut and paste DNA in genetic engineering.
  This implies the manipulation of DNA sequences.
**Method & Steps**:
 1. Identify the enzyme responsible for 'cutting' DNA.
  Reasoning: Restriction enzymes recognize specific DNA sequences and cleave the DNA at those sites.
  Formula/Rule:  None directly applicable, but understanding the function of restriction enzymes is key.
 2. Identify the enzyme responsible for 'pasting' DNA.
  Working: DNA ligase joins the cut DNA fragments by forming phosphodiester bonds between the sugar-phosphate backbones.
  Key Point:  Restriction enzymes create sticky ends or blunt ends, facilitating the ligation process by DNA ligase.
 3. Combine the enzymes involved in cutting and pasting.
  Result: Restriction enzymes and DNA ligase are the key enzymes used in genetic engineering for cutting and pasting DNA.
  Verification: This is a standard procedure in molecular biology and genetic engineering techniques.
 ## OPTION ANALYSIS
A ✓: Restriction enzymes and ligase correctly identify the two enzymes involved in cutting and pasting DNA. Restriction enzymes cut the DNA at specific sequences, and DNA ligase joins the fragments.
B :x: DNA polymerase is responsible for DNA replication, not cutting and pasting.
C :x: RNA polymerase is responsible for RNA synthesis, not DNA manipulation.
D :x: Reverse transcriptase synthesizes DNA from an RNA template; it's not directly involved in cutting and pasting DNA.
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing DNA polymerase with ligase: Remember polymerase replicates, while ligase joins fragments.
  2.Omitting one of the enzymes: Both restriction enzymes and ligase are required for the complete process.
 **Quick Tips**:
  1.Think of restriction enzymes as 'molecular scissors' and ligase as 'molecular glue'.
  2.Remember the key functions of each enzyme to avoid confusion.

D VINOD · Answer

The correct Option is "Restriction enzymes and ligase"

D VINOD · Answer

DNA polymerase

D VINOD · Answer

RNA polymerase

D VINOD · Answer

Reverse transcriptase

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