The Question is from neet-botany biotechnology , and it was created by the author D VINOD

Question

What is the role of a 'reporter gene' in the production of transgenic animals?

D VINOD · Accepted Answer

## CORE INFORMATION
✓ **Answer**: B
## CONCEPT FRAMEWORK
 **Primary Concept**: Transgenic animal technology and gene expression
 **Related Topics**: Molecular biology, genetic engineering, gene regulation
 **Prerequisites**: Understanding of DNA, genes, gene expression, and transgenic organisms.
## SOLUTION PATHWAY
**Given Information**:
The question asks about the role of a reporter gene in creating transgenic animals.

**Method & Steps**:
1. **Understanding Transgenic Animals**: Transgenic animals are organisms that have had foreign DNA (a transgene) introduced into their genome.
2. **The Purpose of a Reporter Gene**:  A reporter gene is a gene whose expression is easily detectable. It's inserted along with the transgene.  The reporter gene's product (e.g., a fluorescent protein or an enzyme) signals successful incorporation of the transgene.
3. **Identifying Successful Transgenesis**: The reporter gene acts as a marker to identify which cells have successfully taken up and expressed the transgene. This is crucial because transgene integration isn't always successful in every cell.
4. **Eliminating Incorrect Options**: Option A is incorrect because the reporter gene isn't usually the gene of interest itself; it's a tool to detect the gene of interest's presence. Option C is incorrect because reporter genes don't directly promote transgene integration; that's the job of other genetic tools. Option D is incorrect because reporter genes typically don't affect the expression of other genes; their role is solely to provide a detectable signal.

**Result**: The reporter gene helps identify cells that have successfully incorporated the transgene.
**Verification**: The definition and function of a reporter gene align with option B.
## OPTION ANALYSIS
A :x: The reporter gene's purpose is not to encode the protein of interest; it's a separate marker gene.
B ✓: This correctly describes the primary function of a reporter gene in transgenic animal production.
C :x: The reporter gene doesn't directly influence the integration of the transgene into the host genome.
D :x: Reporter genes typically don't have a silencing effect on other genes.
## LEARNING AIDS
 **Common Mistakes**:
  1. Confusing the reporter gene with the gene of interest: Remember the reporter gene is a tool to detect successful integration, not the main focus of the experiment.
  2. Overlooking the importance of the reporter gene in identifying successful transgene insertion: The reporter gene is crucial for selecting and studying cells that have successfully incorporated the transgene.
 **Quick Tips**:
  1. Think of a reporter gene as a 'flag' that marks cells containing the transgene.
  2. Common reporter genes include GFP (green fluorescent protein), luciferase, and β-galactosidase.

D VINOD · Answer

It encodes the protein of interest.

D VINOD · Answer

The correct Option is "It helps identify cells that have successfully incorporated the transgene."

D VINOD · Answer

It promotes the integration of the transgene into the host genome.

D VINOD · Answer

It silences the expression of other genes.

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