The Question is from neet-botany molecular-basis-of-inheritance , and it was created by the author B VENKAT

Question

VNTR analysis involves

B VENKAT · Accepted Answer

## CORE INFORMATION
✓ **Answer**: D
## CONCEPT FRAMEWORK
	**Primary Concept**: VNTR Analysis
	**Related Topics**: Restriction Enzymes, DNA Fragmentation, Gel Electrophoresis
	**Prerequisites**: Basic understanding of molecular biology techniques, especially DNA manipulation
## SOLUTION PATHWAY
**Given Information**:
	The question asks about the process of VNTR analysis.
**Method & Steps**:
	1. **Recall Definition of VNTR:** VNTRs (Variable Number Tandem Repeats) are regions of DNA where a short nucleotide sequence is repeated a variable number of times. VNTR analysis relies on differences in the length of these repeated regions between individuals.
	2. **Consider the Process:** VNTR analysis involves cutting DNA with restriction enzymes, which recognize specific DNA sequences. This results in DNA fragments of varying lengths. These fragments are then separated by gel electrophoresis based on their size, creating a unique banding pattern.
	3. **Compare Options:** Option D accurately describes this process.
## OPTION ANALYSIS
[A] :x: Incorrect. VNTRs are not typically two base repeating units.
[B] :x: Incorrect. While some repeats might be 2-4 bp, VNTRs are longer tandem repeats.
[C] :x: Incorrect. PCR is used for amplification, but not the core of VNTR analysis itself. 
[D] ✓: Correct. This accurately describes how VNTR analysis is conducted.
## LEARNING AIDS
	**Common Mistakes**:
		1.[Error 1]: Confusing VNTR with STR (Short Tandem Repeats) analysis. STR uses shorter repeats and often PCR.
		2.[Error 2]: Thinking VNTR analysis directly uses PCR without the restriction digest step.
	**Quick Tips**:
		1.[Helpful shortcut]: Remember the key steps: restriction digestion -> fragment separation (gel electrophoresis).
		2.[Memory aid]: Think of cutting DNA like a puzzle and then sorting the pieces by size.

B VENKAT · Answer

Analyzing specific loci for two base repeating units usually less then 100 bp in size

B VENKAT · Answer

Analyzing specific loci for 2-4 bp repeating units

B VENKAT · Answer

PCR amplification of specific genes

B VENKAT · Answer

The correct Option is "Cutting DNA with restriction enzyme and analyzing the banding pattern of fragments"

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