The Question is from neet-botany , and it was created by the author B VENKAT

Question

Which of the following statement is incorrect w.r.t. pest resistant plants RNA interference?

B VENKAT · Accepted Answer

## CORE INFORMATION
✓ **Answer**: B
## CONCEPT FRAMEWORK
	**Primary Concept**: RNA interference (RNAi) in pest resistant plants
	**Related Topics**: Gene silencing, post-transcriptional gene silencing, Agrobacterium mediated gene transfer, viral RNA genomes
	**Prerequisites**: Basic understanding of molecular biology, central dogma, gene expression, RNA structure
## SOLUTION PATHWAY
**Given Information**:
	* The question asks for the INCORRECT statement about RNAi in pest resistant plants.
	* RNAi is a process used to silence genes
**Method & Steps**:
	1. Analyze each option against known principles of RNAi in pest resistance.
		Reasoning: Evaluate each statement based on knowledge of RNAi mechanisms and their application in plants.
	2. Option A is correct. RNAi is a novel strategy for pest control.
		Reasoning: The technology uses RNAi to target essential genes in pests for silencing. 
	3. Option B is INCORRECT. RNAi involves silencing of mRNA due to a complementary **dsRNA** (double-stranded RNA) molecule, not ssRNA. The dsRNA is processed into smaller fragments, which then guide the silencing complex to the target mRNA.
		Reasoning: The key is the dsRNA origin and its processing into smaller fragments.
	4. Option C is correct. Agrobacterium is a common vector used to introduce genes into plants, including genes for nematode resistance via RNAi.
		Reasoning: Agrobacterium is a standard vector for genetic modification in plants.
	5. Option D is correct. Viral RNA can be a source of dsRNA in RNAi pathways.
		Reasoning: Some viruses produce dsRNA during their replication cycle which can be used in RNAi.
		Result: B is the incorrect statement.
 ## OPTION ANALYSIS
[A] :x: Correct statement about RNAi based pest resistance.
[B] ✓: Incorrect statement. RNAi works via double stranded RNA, not ssRNA
[C] :x: Correct statement about the use of Agrobacterium in plant transformation.
[D] :x: Correct statement about the source of complementary RNA.
 ## LEARNING AIDS
	**Common Mistakes**:
		1. Confusing ssRNA and dsRNA in RNAi: Remember that RNAi is triggered by dsRNA, which is processed into smaller fragments that target mRNA for degradation.
		2. Misunderstanding the source of dsRNA: dsRNA can come from various sources, including viral replication, hairpin RNA, or introduced through genetic engineering.
	**Quick Tips**:
		1. RNAi is a gene silencing mechanism mediated by dsRNA, not ssRNA.
		2. Agrobacterium is a key tool in plant biotechnology for gene delivery.

B VENKAT · Answer

A novel strategy was adopted to prevent pest infestation which was based on the process of RNA interference

B VENKAT · Answer

The correct Option is "RNAi involves silencing of a specific mRNA due to a complementary ssRNA molecule that binds to and prevents translation of mRNA"

B VENKAT · Answer

Agrobacterium vectors have been used to introduce nematode specific genes into the tobacco plant

B VENKAT · Answer

The source of complementary RNA used in this technique could be from an infection by viruses having RNA genomes

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