A researcher uses Agrobacterium tumefaciens to introduce a gene into a plant cell. Which of the following is NOT a crucial step in this process?
Infection of plant cells with the engineered Agrobacterium
Insertion of the gene of interest into the Ti plasmid
Ligation of the gene into a yeast expression vector
Regeneration of the transformed plant cells
Related Questions
A researcher uses Agrobacterium tumefaciens to introduce a gene into a plant cell. Which of the following is NOT a crucial step in this process?
Infection of plant cells with the engineered Agrobacterium
Insertion of the gene of interest into the Ti plasmid
Ligation of the gene into a yeast expression vector
Regeneration of the transformed plant cells
The organism, which is used for gene transfer in higher organisms is
Agrobacterium tumefaciens
E. coli
Acetobacter aceti
Bacillus thuringiensis
The vector used to transfer a gene to produce pest-resistant tobacco plant is
pBR322
pUC8
Modified Ti plasmid
Simian virus
Which technique is used to introduce foreign genes into plant cells?
Gene gun
Centrifugation
Chromatography
Electrophoresis
The vector used to transfer a gene to produce pest-resistant tobacco plant is
pBR322
pUC8
Modified Ti plasmid
Simian virus
Which technique is LEAST suitable for introducing a foreign gene into a mature plant cell with a thick cell wall?
Biolistics
Agrobacterium-mediated transformation
Microinjection
Heat shock transformation
Which of the following vector is commonly used in transfer of gene of interest into the crop plants
Agrobacterium tumefaciens
trichoderma polysporum
Monascus purpureus
Penicillium notatum
The introduction of the Bt gene into cotton plants is an example of which biotechnological application?
Genetic engineering
Tissue culture
Cloning
Hybridization
Which of the following vector is commonly used in transfer of gene of interest into the crop plants
Agrobacterium tumefaciens
trichoderma polysporum
Monascus purpureus
Penicillium notatum
