A researcher uses Agrobacterium tumefaciens to introduce a gene into a plant cell. Which of the following is NOT a crucial step in this process?
Infection of plant cells with the engineered Agrobacterium
Insertion of the gene of interest into the Ti plasmid
Ligation of the gene into a yeast expression vector
Regeneration of the transformed plant cells
Related Questions
Which of the following statement is incorrect w.r.t. pest resistant plants RNA interference?
A novel strategy was adopted to prevent pest infestation which was based on the process of RNA interference
RNAi involves silencing of a specific mRNA due to a complementary ssRNA molecule that binds to and prevents translation of mRNA
Agrobacterium vectors have been used to introduce nematode specific genes into the tobacco plant
The source of complementary RNA used in this technique could be from an infection by viruses having RNA genomes
Prior to the fusion process in somatic hybridization, plant cells are treated to remove their:
Cell membranes
Nuclei
Cell walls
Chloroplasts
Applications, like bioremediation, processed food, therapeutics and diagnostics are related with
Biochemistry
Microbiology
Biotechnology
Medical science
Main objective of production/use of herbicide resistant GM crops is to
Encourage eco-friendly herbicides
Reduce herbicide accumulation in food articles for human consumption
Eliminate weeds from the field without the use of manual labor
Select herbicide resistant crops
Consider the following statements about transgenic tobacco plant
I. Transgenic tobacco plants contains a gene from a bacterium,
II. gene is an insecticidal protein which damages the inner lining of the insects and kills it (insect)
III. The tobacco plants having gene produces their own insecticide
Which of the statements given above are correct?
I and II
I and III
II and III
I, II and III
Disadvantages of chemical agents are
I. chemicals are toxic and harmful to human beings and animals
II. chemical pollute the environment and plants
III. weedicides used to remove weeds also pollute the soil
Choose the correct option
I, II and III
I and II
I and III
II and III
Consider the following statements
I. Bt toxin gene has been cloned from the bacteria
II. Genetic engineering works only on animals and has not yet been successfully used on plants
III. Strains of Bacillus thuringiensis are used in producing bioinsecticidal plants
Which of the statements given above are correct?
I and II
I and III
II and III
I, II and III
toxin protein crystals present in bacterium Bacillus thuringiensis, do not kill the bacteria themselves because
Bacteria are resistant to the toxin
Bacteria enclose toxins in a special sac
Toxins occur an inactive protoxins in bacteria
None of the above
Flavr savr variety of tomato, remains fresh for a longer period than normal tomato due to
Presence of pest resistance gene
Over expression of gene coding for enzyme polygalacturonase
Silencing of gene coding for enzyme polygalacturonase
Presence of vitamin A and C
Consider the following statements about organic farming
I. Organic farming promotes the use of crop rotations and cover crops and encourages balanced host/predator relationships
II. Integrated pest and weed management and soil conservation systems are valuable tools on an organic farm
II. Organic farming protects the environment, minimize soil degradation and erosion and decrease pollution
Which of the statements given above are correct?
I, II and III
I and II
I and III
II and III
