A researcher uses Agrobacterium tumefaciens to introduce a gene into a plant cell. Which of the following is NOT a crucial step in this process?
Infection of plant cells with the engineered Agrobacterium
Insertion of the gene of interest into the Ti plasmid
Ligation of the gene into a yeast expression vector
Regeneration of the transformed plant cells
Related Questions
Which of the following describes the mechanism of action of Bt toxin coded by the cry gene?
Active toxin is ingested and directly kills the insect.
Inactive protoxin is ingested and activated by the alkaline pH of the insect gut.
Active toxin is ingested and deactivated by the gut pH, then reactivated later.
Inactive protoxin is ingested and activated by enzymes in the insect's saliva.
Which of the following is NOT a benefit associated with golden rice?
Increased Vitamin A intake
Reduced Vitamin A deficiency
Increased iron content
Improved nutritional value
Which combination of technologies is MOST likely to be employed in developing a 'Golden Rice' variety with enhanced iron bioavailability?
Phytoene synthase gene transfer, Lycopene beta-cyclase gene transfer
Ferritin gene transfer, Phytase gene transfer
Cry gene transfer, Proteinase inhibitor gene transfer
Viral coat protein gene transfer, Chitinase gene transfer
The water soluble protein associated with silk thread is
Fibroin
Sericin
Chitin
Mucin
Which technique is LEAST suitable for introducing a foreign gene into a mature plant cell with a thick cell wall?
Biolistics
Agrobacterium-mediated transformation
Microinjection
Heat shock transformation
Flavr savr variety of tomato, remains fresh for a longer period than normal tomato due to
Presence of pest resistance gene
Over expression of gene coding for enzyme polygalacturonase
Silencing of gene coding for enzyme polygalacturonase
Presence of vitamin A and C
Golden rice is a genetically modified crop plant where the incorporated gene is meant for the biosynthesis of:
Vitamin A precursor
Vitamin B
Vitamin C
Vitamin D
A scientist is attempting to develop a transgenic crop resistant to a specific lepidopteran pest. Which gene would be the LEAST suitable candidate for transfer?
A gene encoding a Bt toxin
A gene conferring resistance to a bacterial pathogen
A gene involved in photosynthesis
A gene encoding a protein that disrupts insect gut function
Why doesn't the Bt toxin produced by Bacillus thuringiensis harm the bacterium itself?
The bacterium has a special enzyme that degrades the toxin.
The bacterium's cell wall is impermeable to the toxin.
It exists as an inactive protoxin within the bacterium.
The bacterium produces an antitoxin that neutralizes the Bt toxin.
The cryIAc gene codes for a Bt toxin that targets specific insect groups. Which of the following is TRUE regarding the activation of this toxin?
It is activated by the acidic pH of the insect gut.
It is activated by the alkaline pH of the insect gut.
It is activated by enzymes in the insect's saliva.
It is always active and does not require activation.
