The Question is from neet-botany biotechnology , and it was created by the author Mr. NARESH KUMAR K

Question

Which of the following statement is incorrect w.r.t. pest resistant plants RNA interference?

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: A
## CONCEPT FRAMEWORK
	**Primary Concept**: RNA interference (RNAi) mechanism for pest resistance in plants.
	**Related Topics**: Gene silencing, dsRNA, siRNA, mRNA degradation, plant biotechnology.
	**Prerequisites**: Basic understanding of gene expression and molecular biology.
## SOLUTION PATHWAY
**Given Information**:
	The question asks for the INCORRECT statement regarding RNAi in pest-resistant plants.
**Method & Steps**:
	1. **Analyze each option based on the RNAi mechanism:**
		Reasoning: RNAi involves the use of dsRNA to silence specific genes.
	2. **Identify the step where the incorrect information lies:**
		Working: Option A mentions methylation of target DNA, which is not the primary mechanism of RNAi.
		Key Point: RNAi primarily works by degrading mRNA of the target gene, not through DNA methylation.
	3. **Confirm the correct mechanism of RNAi:**
		Result: RNAi involves dsRNA being processed into siRNA, which then guides the degradation of the target mRNA.
		Verification: Options B, C, and D accurately describe the RNAi mechanism and its application in pest-resistant plants.
## OPTION ANALYSIS
[A] :x: RNAi silences genes by degrading the mRNA of the pest, not by methylation of the target DNA sequence. DNA methylation is a separate gene silencing mechanism.
[B] ✓: The dsRNA expressed in the plant is designed to be specific to a vital gene of the insect pest, ensuring that the RNAi effect is targeted.
[C] ✓: The dsRNA is processed into smaller interfering RNAs (siRNAs), which then guide the degradation of the complementary mRNA of the pest, effectively silencing the target gene.
[D] ✓: RNAi technology has been successfully used to develop pest-resistant plants in several crops, including cotton and tobacco.
## LEARNING AIDS
	**Common Mistakes**:
		1. Confusing RNAi with other gene silencing mechanisms like DNA methylation: Remember that RNAi primarily acts at the mRNA level.
		2. Misunderstanding the role of dsRNA and siRNA: dsRNA is the trigger, but siRNA is the actual effector molecule that guides mRNA degradation.
	**Quick Tips**:
		1. Remember the central dogma: DNA -> RNA -> Protein. RNAi interferes at the RNA level.
		2.  Visualize the process: dsRNA -> siRNA -> mRNA degradation.

Mr. NARESH KUMAR K · Answer

The correct Option is "RNAi silences the pest target genes by methylation of the target DNA sequence."

Mr. NARESH KUMAR K · Answer

The dsRNA expressed in the plant is specific to a vital gene of the insect pest.

Mr. NARESH KUMAR K · Answer

The dsRNA is processed into siRNA which then degrades the mRNA of the pest.

Mr. NARESH KUMAR K · Answer

RNAi technique has been used to develop pest-resistant plants in several crops like cotton and tobacco.

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