The Question is from neet-botany biotechnology , and it was created by the author K SURESH KUMAR

Question

RNA interference (RNAi) technology is being explored for developing pest-resistant plants. Which statement about RNAi is INCORRECT?

K SURESH KUMAR · Accepted Answer

## CORE INFORMATION
✓ **Answer**: D
## CONCEPT FRAMEWORK
	**Primary Concept**: RNA interference (RNAi)
	**Related Topics**: Gene silencing, Pest control, Biotechnology, Plant breeding
	**Prerequisites**: Basic understanding of DNA, RNA, and gene expression.
## SOLUTION PATHWAY
**Given Information**:
	The question asks for the INCORRECT statement regarding RNAi technology in pest-resistant plants.
**Method & Steps**:
	1. Analyze each option based on the mechanism of RNAi and its application in plants.
	2. Identify the statement that misrepresents the nature or inheritance of the RNAi trait.
	3. Confirm the correct option by verifying the accuracy of the other options.
## OPTION ANALYSIS
[A] ✓: The RNAi effect can be highly specific to the target pest.  RNAi targets specific mRNA sequences. Careful design of the dsRNA can ensure that it only affects the target pest and not other organisms, including the plant itself. This specificity is a major advantage of RNAi technology.
[B] ✓: The introduced dsRNA is processed into smaller interfering RNAs (siRNAs). This is a core step in the RNAi pathway. The introduced double-stranded RNA (dsRNA) is cleaved by the enzyme Dicer into siRNAs, which are then incorporated into the RNA-induced silencing complex (RISC) to guide the degradation of target mRNA.
[C] ✓: RNAi primarily works by blocking translation of target mRNA.  The siRNAs guide the RISC to complementary mRNA molecules. The RISC then cleaves the target mRNA, preventing its translation into protein. This effectively silences the gene and disrupts the pest's biological processes.
[D] :x: The RNAi trait is easily inherited through traditional plant breeding methods.  While the gene encoding the dsRNA can be inherited, the inheritance patterns can be complex and may not always result in stable expression of the dsRNA in subsequent generations.  Traditional breeding involves crossing plants and selecting offspring with desired traits.  The integration site of the transgene producing the dsRNA can influence its inheritance and expression levels.  Furthermore, multiple copies of the transgene can be inserted, leading to unpredictable segregation patterns in offspring. Therefore, ensuring stable inheritance of the RNAi trait often requires more advanced genetic engineering approaches rather than simple traditional breeding.
## LEARNING AIDS
	**Common Mistakes**:
		1. Confusing RNAi with other gene editing techniques:  RNAi is a gene silencing mechanism, not a gene editing technique like CRISPR. It doesn't alter the DNA sequence itself but rather interferes with the expression of existing genes.
		2. Assuming all genetic modifications are easily inherited: The inheritance of transgenes, including those for RNAi, can be complex and depends on several factors, including the integration site and the number of copies inserted.
	**Quick Tips**:
		1. Remember the key steps of RNAi: dsRNA → siRNA → RISC → mRNA degradation.
		2. Consider the target specificity of RNAi as a major advantage in pest control.

K SURESH KUMAR · Answer

The RNAi effect can be highly specific to the target pest.

K SURESH KUMAR · Answer

The introduced dsRNA is processed into smaller interfering RNAs (siRNAs).

K SURESH KUMAR · Answer

RNAi primarily works by blocking translation of target mRNA.

K SURESH KUMAR · Answer

The correct Option is "The RNAi trait is easily inherited through traditional plant breeding methods."

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