The Question is from neet-botany molecular-basis-of-inheritance dna-finger-printing, and it was created by the author Mr. NARESH KUMAR K

Question

Which technique is used to transfer DNA fragments from an electrophoresis gel to a nylon membrane during DNA fingerprinting?

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: [C]
## CONCEPT FRAMEWORK
	**Primary Concept**: Molecular Biology Techniques
	**Related Topics**: Gel Electrophoresis, DNA Fingerprinting, Blotting Techniques
	**Prerequisites**: Basic understanding of DNA structure and function, gel electrophoresis principles
## SOLUTION PATHWAY
**Given Information**:
	* DNA fragments are separated by electrophoresis.
	* Need to transfer DNA from the gel to a membrane.
**Method & Steps**:
	1. **Identify the process**: DNA fragments, separated by electrophoresis, need to be transferred to a solid support (membrane) for further analysis.
		Reasoning: This transfer process is a specific type of blotting technique.
		Formula/Rule: N/A
	2. **Determine the correct blot**:  We need to identify the blotting technique that specifically transfers DNA.
		Working: Southern blotting is specifically used for DNA transfer, while Northern blotting is for RNA and Western blotting for proteins.
		Key Point: Each blotting technique targets different biomolecules. Eastern blotting is not a standard technique and is less commonly used.
	3. **Final Answer:** The correct technique is Southern Blotting.
		Result: Southern Blotting
		Verification: Confirmed by the function of the technique
 ## OPTION ANALYSIS
[A] :x: Northern Blotting is used for RNA transfer, not DNA.
[B] :x: Western Blotting is used for protein transfer, not DNA.
[C] ✓: Southern Blotting is the correct technique used to transfer DNA from a gel to a membrane.
[D] :x: Eastern Blotting is not a standard technique and it does not transfer DNA.
 ## LEARNING AIDS
	**Common Mistakes**:
		1.[Error 1]: Confusing the different blotting techniques. [How to avoid]: Memorize which technique targets which biomolecule (S-DNA, N-RNA, W-Protein).
		2.[Error 2]: Thinking that Eastern Blotting is a standard technique. [How to avoid]: Recognize that it is less commonly used and not related to DNA transfer.
	**Quick Tips**:
		1.[Helpful shortcut]: Use the mnemonic 'SNOW DROP' for Southern-DNA, Northern-RNA, Western-Protein.
		2.[Memory aid]: Remember 'Southern' for 'DNA' as both start with 'S'.

Mr. NARESH KUMAR K · Answer

Northern Blotting

Mr. NARESH KUMAR K · Answer

Western Blotting

Mr. NARESH KUMAR K · Answer

The correct Option is "Southern Blotting"

Mr. NARESH KUMAR K · Answer

Eastern Blotting

NEET Botany Molecular Basis of Inheritance Dna Finger Printing MCQs

Select Topic

Question Tags

Question Tags

Question Tags

Question Tags

Question Tags

Question Tags

Question Tags

Question Tags

Question Tags

Question Tags

NEET Subjects

Our Bots

Company