The Question is from neet-botany molecular-basis-of-inheritance human-genome-project, and it was created by the author NISHANTH

Question

The Human Genome Project utilized a hierarchical approach to sequencing. Which of the following correctly describes the 'clone-by-clone' sequencing strategy?

NISHANTH · Accepted Answer

## CORE INFORMATION
✓ **Answer**: D
## CONCEPT FRAMEWORK
 **Primary Concept**: Genome Sequencing Strategies
 **Related Topics**: Human Genome Project, BACs, Cloning, DNA Sequencing
 **Prerequisites**: Understanding of DNA structure, cloning techniques, and sequencing technologies.
## SOLUTION PATHWAY
**Given Information**:
The question asks about the clone-by-clone sequencing strategy used in the Human Genome Project.
**Method & Steps**:
1. Understanding the clone-by-clone approach:
  Reasoning: The clone-by-clone strategy is a hierarchical approach where the genome is broken down into manageable fragments for sequencing.
  Formula/Rule:  No specific formula is required.
2. Analyzing the options:
  Working: Let's evaluate each option:
  Option A: This describes a shotgun sequencing approach, not clone-by-clone.
  Option B: This also describes a shotgun sequencing approach.
  Option C: This is not a common or efficient approach for large-scale genome sequencing.
  Option D: This accurately describes the clone-by-clone method: large DNA fragments are first cloned into Bacterial Artificial Chromosomes (BACs), then mapped to determine their order, and finally subcloned into smaller vectors for easier sequencing.
  Key Point: BACs are large cloning vectors capable of holding large DNA fragments.
3. Final Step:
  Result: Option D correctly describes the clone-by-clone sequencing strategy.
  Verification: The description matches the characteristics of the clone-by-clone approach used in the Human Genome Project.
 ## OPTION ANALYSIS
A :x: This describes a whole-genome shotgun sequencing approach.
B :x: This describes a whole-genome shotgun sequencing approach.
C :x: This is not a feasible or efficient strategy for sequencing a large genome.
D ✓: This accurately describes the clone-by-clone strategy, which involves cloning large fragments into BACs, mapping them, and then sequencing subclones.
 ## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing clone-by-clone with shotgun sequencing:  Understanding the fundamental differences between these approaches is crucial.
  2.Lack of knowledge about BACs and their role in large-scale genome sequencing: Familiarize yourself with the properties and uses of BACs and other cloning vectors.
 **Quick Tips**:
  1.Remember that clone-by-clone is a hierarchical approach, proceeding from large to small fragments.
  2.Associate BACs with clone-by-clone sequencing.

NISHANTH · Answer

Large DNA fragments were directly sequenced using high-throughput methods.

NISHANTH · Answer

Small DNA fragments were randomly sequenced and assembled computationally.

NISHANTH · Answer

The entire genome was amplified using PCR and then sequenced in segments.

NISHANTH · Answer

The correct Option is "Large DNA fragments were cloned into BACs, mapped, and then subcloned into smaller vectors for sequencing."

NEET Botany Molecular Basis of Inheritance Human Genome Project MCQs

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