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NEET Questions / Botany / Biotechnology and Its Applications / Genetically Engineered Insulin
Which specific enzymatic cleavage is crucial for converting proinsulin to active insulin in the production of genetically engineered human insulin?
A. Cleavage at the N-terminus of the A-chain
B. Cleavage at the C-terminus of the B-chain
C. Removal of the C-peptide by specific endopeptidases
D. Cleavage of disulfide bonds linking A and B chains
In the context of producing genetically engineered human insulin, why is the formation of inclusion bodies in E. coli sometimes considered advantageous despite requiring additional processing steps?
A. Inclusion bodies protect insulin from proteolytic degradation within the bacterial cell
B. Inclusion bodies directly facilitate the formation of correct disulfide bonds
C. Inclusion bodies enhance the solubility and secretion of insulin from the bacterial cell
D. Inclusion bodies simplify the purification process by eliminating the need for chromatography
Regarding insulin, which of the following statements are correct?
(a) Mature insulin lacks C-peptide.
(b) Insulin produced via rDNA technology contains C-peptide.
(c) Pro-insulin has C-peptide.
(d) Disulfide bridges link the A-peptide and B-peptide of insulin.
(a), (b), (c)
(a), (c), (d)
(b), (c), (d)
(a), (b), (d)
Which of the following statements is incorrect regarding insulin produced by rDNA technology?
It is readily available.
It is identical to human insulin.
It avoids ethical concerns associated with animal-derived insulin.
It always contains C-peptide.
Which of the following statements is/are TRUE regarding the production of human insulin using recombinant DNA technology?
It involves the use of animal pancreatic tissue.
The insulin produced is slightly different from human insulin.
It is a very expensive and inefficient process.
It allows for large-scale production of human insulin.
Read the following statements about insulin and choose the option that states all correct statements.
a. Earlier, insulin was extracted from pancreas of slaughtered cattles and pigs
b. Mature insulin do not contain polypeptide C
c. Mature insulin consists of two short polypeptides: chain A and chain B
d. During commercial production of insulin, chain A and B were produced together, extracted and combined by creating disulphide bonds
a, b and c
a, b, c and d
a, c and d
b, c and d
Read the following statements about insulin and choose the option that states all correct statements.
a. Earlier, insulin was extracted from pancreas of slaughtered cattles and pigs
b. Mature insulin do not contain polypeptide C
c. Mature insulin consists of two short polypeptides: chain A and chain B
d. During commercial production of insulin, chain A and B were produced together, extracted and combined by creating disulphide bonds
a, b and c
b, c and d
a, c and d
a, b and d
Which of the following statements are false?
I. Insulin for curing diabetes, used to be extracted from the pancreas of slaughtered pig and cattle
II. Animal insulin is slighty different from the human insulin
III. Animal insulin causes some undesirable side effects such as allergy
IV. Bacteria cannot be made to synthesise insulin from its gene because of the presence of introns
Choose the correct option
I and II only
II and III only
III and IV only
IV only
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
