The Question is from neet-botany molecular-basis-of-inheritance , and it was created by the author Mr. NARESH KUMAR K

Question

During DNA replication in eukaryotes, the lagging strand is synthesized discontinuously as Okazaki fragments. Which enzyme is primarily responsible for joining these fragments together after the removal of RNA primers?

Mr. NARESH KUMAR K · Accepted Answer

## CORE INFORMATION
✓ **Answer**: [Option D]
## CONCEPT FRAMEWORK
    **Primary Concept**: DNA replication and the role of enzymes in the process
    **Related Topics**: Enzymes involved in DNA synthesis, Okazaki fragments, leading and lagging strands
    **Prerequisites**: Knowledge of DNA structure, replication process, and enzyme functions
## SOLUTION PATHWAY
**Given Information**:
        - Lagging strand synthesized discontinuously as Okazaki fragments
        - RNA primers are removed during the process
        - Enzymes involved in DNA replication include DNA polymerases and ligase
**Method & Steps**:
    1.    Identify the role of different enzymes in DNA replication.
        Reasoning: Each enzyme has a specific function, and it's essential to pinpoint which one connects Okazaki fragments.
        Formula/Rule: None applicable.
    2.    Recognize that after RNA primers are removed, the fragments need to be joined.
        Working: This joining is a critical step in completing the lagging strand synthesis.
        Key Point: DNA Ligase is known to join DNA fragments by forming phosphodiester bonds.
    3.    Conclude that DNA Ligase is responsible for the final step of joining the Okazaki fragments.
        Result: The enzyme that joins the fragments is DNA Ligase.
        Verification: Cross-reference with standard molecular biology texts or resources to confirm the role of DNA Ligase.
## OPTION ANALYSIS
[A] :x: DNA Polymerase I primarily removes RNA primers but does not join fragments.
[B] :x: DNA Polymerase III synthesizes new DNA but does not join fragments.
[C] :x: DNA Primase synthesizes RNA primers but does not join Okazaki fragments.
[D] ✓: DNA Ligase is the correct enzyme responsible for joining Okazaki fragments together.
## LEARNING AIDS
    **Common Mistakes**:
        1. Confusing the roles of different DNA polymerases: Remember that Polymerase I removes RNA primers, while Polymerase III synthesizes DNA.
        2. Overlooking the function of DNA Ligase: It's important to remember that ligase connects fragments, not synthesizes them.
    **Quick Tips**:
        1. Remember "L" in Ligase for "Joining" (Ligating) fragments.
        2. Use the acronym PRIMER to recall the order of RNA primer removal (P: Primase, R: RNA Primer, I: I for DNA Polymerase I, M: for the joining process, E: Enzyme Ligase, R: Resulting DNA strand).

Mr. NARESH KUMAR K · Answer

DNA Polymerase I

Mr. NARESH KUMAR K · Answer

DNA Polymerase III

Mr. NARESH KUMAR K · Answer

DNA Primase

Mr. NARESH KUMAR K · Answer

The correct Option is "DNA Ligase"

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