The Question is from neet-botany molecular-basis-of-inheritance , and it was created by the author B VENKAT

Question

Eukaryotic DNA replication is a complex process involving multiple proteins. Which of the following is NOT a function of Replication Protein A (RPA)?

B VENKAT · Accepted Answer

## CORE INFORMATION
✓ **Answer**: D
## CONCEPT FRAMEWORK
 **Primary Concept**: DNA Replication in Eukaryotes
 **Related Topics**: DNA polymerase, single-stranded binding proteins, DNA replication enzymes, Okazaki fragments
 **Prerequisites**: Understanding of the basic steps in DNA replication and the roles of key enzymes.
## SOLUTION PATHWAY
**Given Information**:
The question asks about the functions of Replication Protein A (RPA).
**Method & Steps**:
1. Understand the role of RPA in DNA replication.
  Reasoning: RPA is a single-stranded DNA-binding protein (SSB). SSBs are crucial for stabilizing the single-stranded DNA that is generated during the unwinding of the double helix. This prevents the single strands from reannealing (coming back together) and also allows other replication proteins to bind and carry out their functions.
  Formula/Rule: None directly applicable.
2. Analyze the given options.
  Working: Options A, B, and C describe known functions of RPA. Option D, catalyzing the formation of phosphodiester bonds, is the function of DNA polymerase.
  Key Point: RPA is a single-stranded DNA-binding protein; it does not possess enzymatic activity.
3. Identify the incorrect function.
  Result: Option D is the correct answer, as RPA does not catalyze the formation of phosphodiester bonds.
  Verification: The function of forming phosphodiester bonds is the primary function of DNA polymerase, which links nucleotides together to create the new DNA strand.
## OPTION ANALYSIS
A ✓: Stabilizing single-stranded DNA - This is a core function of RPA.
B :x: Preventing reannealing of separated DNA strands - This is another key function of RPA.
C :x: Facilitating the binding of other replication proteins - This is also a key function of RPA, acting as a platform for other proteins.
D :x: Catalyzing the formation of phosphodiester bonds - This is the function of DNA polymerase, not RPA.
## LEARNING AIDS
 **Common Mistakes**:
  1.Confusing RPA with DNA polymerase: Remember that RPA is a binding protein, not an enzyme.
  2.Overlooking the role of RPA in facilitating the binding of other proteins: RPA's role in coordinating the replication process is vital.
 **Quick Tips**:
  1.Remember the acronym RPA (Replication Protein A) and associate it with its primary function of stabilizing single-stranded DNA.
  2.Understand the difference between binding proteins and enzymes in DNA replication.

B VENKAT · Answer

Stabilizing single-stranded DNA

B VENKAT · Answer

Preventing reannealing of separated DNA strands

B VENKAT · Answer

Facilitating the binding of other replication proteins

B VENKAT · Answer

The correct Option is "Catalyzing the formation of phosphodiester bonds"

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