Prepare for NEET Biology Molecular Basis of Inheritance with MCQs & PYQs on NEET.GUIDE. Access free practice, previous year questions, and expert explanations of DNA replication and genetics.
Which of the following is NOT a function of the release factor during translation?
Recognizing stop codons
Hydrolyzing the bond between the polypeptide and the tRNA
Promoting the dissociation of the ribosomal subunits
Catalyzing the formation of a peptide bond
A mutation in the 16S rRNA of the prokaryotic ribosome could directly affect which stage of translation?
Elongation
Termination
Initiation
All stages
In DNA fingerprinting, the process of separating DNA fragments of different sizes is called:
Polymerase Chain Reaction
Electrophoresis
Southern Blotting
Hybridization
Variable Number Tandem Repeats (VNTRs) are crucial in DNA fingerprinting because they:
Are highly conserved sequences found in all individuals
Code for essential proteins involved in DNA replication
Show high variability in their number of repeats between individuals
Are only found in specific tissues
Which technique is used to transfer DNA fragments from an electrophoresis gel to a nylon membrane during DNA fingerprinting?
Northern Blotting
Western Blotting
Southern Blotting
Eastern Blotting
Which enzyme is used to cut DNA at specific sequences during DNA fingerprinting?
DNA polymerase
RNA polymerase
Ligase
Restriction endonuclease
The unique pattern of DNA fragments obtained in DNA fingerprinting is due to:
Variations in the coding sequences of genes
Variations in the length of VNTR sequences
Differences in the number of chromosomes
Mutations in mitochondrial DNA
In DNA fingerprinting, probes are used to:
Cut DNA at specific sites
Amplify the entire DNA sample
Separate DNA fragments by size
Hybridize with specific VNTR sequences
Why is the use of mitochondrial DNA (mtDNA) preferred over nuclear DNA in forensic analysis of highly degraded samples?
Longer sequence length
Greater polymorphism
Higher copy number per cell
Absence of introns
Which of the following techniques is NOT directly involved in generating a DNA fingerprint?
Restriction Fragment Length Polymorphism (RFLP) analysis
Southern blotting
Polymerase Chain Reaction (PCR) using specific primers
Polymerase Chain Reaction (PCR) using random primers
