The Question is from neet-botany , and it was created by the author D VINOD

Question

During DNA replication, Okazaki fragments are used to enlongate

D VINOD · Accepted Answer

## CORE INFORMATION
✓ **Answer**: A
## CONCEPT FRAMEWORK
**Primary Concept**: DNA replication mechanism and the role of Okazaki fragments.
**Related Topics**: Leading strand synthesis, lagging strand synthesis, DNA polymerase, replication fork.
**Prerequisites**: Basic knowledge of DNA structure and replication.
## SOLUTION PATHWAY
**Given Information**:
Okazaki fragments are involved in DNA replication.
**Method & Steps**:
1. **Recall the directionality of DNA synthesis**: DNA polymerase can only add nucleotides to the 3' end of a growing strand. Therefore, DNA synthesis proceeds in a 5' to 3' direction.
2. **Understand the leading and lagging strands**: Because the two strands of DNA are antiparallel, one strand (leading strand) can be synthesized continuously in the 5' to 3' direction towards the replication fork. The other strand (lagging strand) must be synthesized discontinuously in short fragments (Okazaki fragments) in the 5' to 3' direction away from the replication fork.
3. **Identify the role of Okazaki fragments**: Okazaki fragments are short DNA fragments synthesized on the lagging strand.
4. **Relate direction of synthesis to Okazaki fragments**: Since the lagging strand is synthesized away from the replication fork, Okazaki fragments are used to elongate the lagging strand away from the replication fork.
## OPTION ANALYSIS
[A] ✓: The lagging strand is synthesized discontinuously using Okazaki fragments and is oriented away from the replication fork.
[B] :x: The leading strand is synthesized continuously, not using Okazaki fragments, and is oriented towards the replication fork.
[C] :x: While Okazaki fragments are used in lagging strand synthesis, they are oriented away from the replication fork, not towards it.
[D] :x: The leading strand is synthesized continuously and is oriented towards the replication fork.
## LEARNING AIDS
**Common Mistakes**:
1. **Confusing leading and lagging strands**: Remember that the leading strand is synthesized continuously, and the lagging strand is synthesized discontinuously.
2. **Misunderstanding the direction of DNA synthesis**: DNA polymerase always adds nucleotides to the 3' end, leading to 5' to 3' synthesis direction.
**Quick Tips**:
1. Visualize the replication fork and the direction of DNA synthesis on each strand to easily identify the leading and lagging strand.
2. Remember that Okazaki fragments are always associated with lagging strand synthesis.

D VINOD · Answer

The correct Option is "The lagging strand away from the replication fork"

D VINOD · Answer

The leading strand towards replication fork

D VINOD · Answer

The lagging strand towards replication fork

D VINOD · Answer

The leading strand away from replication fork

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