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NEET Questions / Botany / Biotechnology and Its Applications
The production of human insulin in bacteria involves the insertion of the human gene for insulin into a bacterial plasmid. This plasmid then acts as a:
Vector
Promoter
Operator
Repressor
What is an advantage of using genetically engineered human insulin over insulin extracted from animal sources?
Reduced risk of allergic reactions
Lower production cost
Higher insulin potency
Longer shelf life
Which of the following statements is/are TRUE regarding the production of human insulin using recombinant DNA technology?
It involves the use of animal pancreatic tissue.
The insulin produced is slightly different from human insulin.
It is a very expensive and inefficient process.
It allows for large-scale production of human insulin.
In the production of recombinant human insulin, which of the following is NOT a step involved?
Inserting the human insulin gene into E. coli
Culturing the E. coli to produce insulin chains
Extracting insulin directly from animal pancreases
Joining the A and B chains of insulin through disulfide bonds
Why is the production of human insulin using recombinant DNA technology preferred over extracting it from animals?
It is cheaper to produce
Animals produce insufficient quantities of insulin
Reduces the risk of allergic reactions and is more ethical
Bacteria produce more potent insulin
Read the following statements about insulin and choose the option that states all correct statements.
a. Earlier, insulin was extracted from pancreas of slaughtered cattles and pigs
b. Mature insulin do not contain polypeptide C
c. Mature insulin consists of two short polypeptides: chain A and chain B
d. During commercial production of insulin, chain A and B were produced together, extracted and combined by creating disulphide bonds
a, b and c
a, b, c and d
a, c and d
b, c and d
Read the following statements about insulin and choose the option that states all correct statements.
a. Earlier, insulin was extracted from pancreas of slaughtered cattles and pigs
b. Mature insulin do not contain polypeptide C
c. Mature insulin consists of two short polypeptides: chain A and chain B
d. During commercial production of insulin, chain A and B were produced together, extracted and combined by creating disulphide bonds
a, b and c
b, c and d
a, c and d
a, b and d
Which of the following statements are false?
I. Insulin for curing diabetes, used to be extracted from the pancreas of slaughtered pig and cattle
II. Animal insulin is slighty different from the human insulin
III. Animal insulin causes some undesirable side effects such as allergy
IV. Bacteria cannot be made to synthesise insulin from its gene because of the presence of introns
Choose the correct option
I and II only
II and III only
III and IV only
IV only
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
Splitting A and B-peptide chain
Addition of C-peptide to proinsulin
Getting insulin assembled into mature form
Removal of C-peptide from active insulin
