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NEET Questions / Botany / Biotechnology and Its Applications
A researcher uses Agrobacterium tumefaciens to introduce a gene into a plant cell. Which of the following is NOT a crucial step in this process?
Infection of plant cells with the engineered Agrobacterium
Insertion of the gene of interest into the Ti plasmid
Ligation of the gene into a yeast expression vector
Regeneration of the transformed plant cells
Which technique is LEAST suitable for introducing a foreign gene into a mature plant cell with a thick cell wall?
Biolistics
Agrobacterium-mediated transformation
Microinjection
Heat shock transformation
Which gene transfer method is commonly used to create transgenic plants?
Electroporation
Microinjection
Agrobacterium-mediated transformation
Particle bombardment
Which of the following vector is commonly used in transfer of gene of interest into the crop plants
Agrobacterium tumefaciens
trichoderma polysporum
Monascus purpureus
Penicillium notatum
Which of the following vector is commonly used in transfer of gene of interest into the crop plants
Agrobacterium tumefaciens
trichoderma polysporum
Monascus purpureus
Penicillium notatum
Which of the following statement is incorrect w.r.t. pest resistant plants RNA interference?
RNAi silences the pest target genes by methylation of the target DNA sequence.
The dsRNA expressed in the plant is specific to a vital gene of the insect pest.
The dsRNA is processed into siRNA which then degrades the mRNA of the pest.
RNAi technique has been used to develop pest-resistant plants in several crops like cotton and tobacco.
Which of the following statement is incorrect w.r.t. pest resistant plants RNA interference?
A novel strategy was adopted to prevent pest infestation which was based on the process of RNA interference
RNAi involves silencing of a specific mRNA due to a complementary ssRNA molecule that binds to and prevents translation of mRNA
Agrobacterium vectors have been used to introduce nematode specific genes into the tobacco plant
The source of complementary RNA used in this technique could be from an infection by viruses having RNA genomes
The organism, which is used for gene transfer in higher organisms is
Agrobacterium tumefaciens
E. coli
Acetobacter aceti
Bacillus thuringiensis
The is often used for making transgenic plants. This plasmid is found in
Azotobacter
Rhizobium of the roots of leguminous plants
Agrobacterium
Yeast as a 2 plasmid
The is often used for making transgenic plants. This plasmid is found in
Azotobacter
Rhizobium of the roots of leguminous plants
Agrobacterium
Yeast as a 2 plasmid
